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navigate/docs/source/05_reference/implementations/configurations/configuration_biofrontiers.yaml
2025-12-04 16:07:30 +08:00

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YAML

microscopes:
OPM:
daq:
hardware:
type: NI
sample_rate: 100000
master_trigger_out_line: PXI6723/port0/line1
camera_trigger_out_line: /PXI6723/ctr0
trigger_source: /PXI6723/PFI0
laser_port_switcher: PXI6723/port0/line0
laser_switch_state: False
camera:
hardware:
type: HamamatsuOrca
serial_number: 000646
defect_correct_mode: 2.0
delay: 10.0 #ms
settle_down: 0.0 #ms
flip_x: False
flip_y: False
remote_focus_device:
hardware:
type: SyntheticRemoteFocus
channel: PXI6723/ao2
min: 0
max: 5
galvo:
-
hardware:
type: NI
channel: PXI6723/ao0
min: -10
max: 10
waveform: sine
phase: 1.57079
filter_wheel:
hardware:
type: ASI
wheel_number: 1
port: COM13
baudrate: 115200
filter_wheel_delay: .030
available_filters:
Empty-Alignment: 0
GFP - FF01-515/30-32: 1
RFP - FF01-595/31-32: 2
Far-Red - BLP01-647R/31-32: 3
Blocked1: 4
Blocked2: 5
Blocked3: 6
Blocked4: 7
Blocked5: 8
Blocked6: 9
stage:
hardware:
-
type: ASI
serial_number: 123456789
axes: [x, y, f]
axes_mapping: [X, Y, Z]
volts_per_micron: None
max: None
min: None
port: COM13
baudrate: 115200
timeout: 0.25
-
type: GalvoNIStage
serial_number: 987654321
axes: [z]
axes_mapping: [ PXI6723/ao1 ]
volts_per_micron: 0.05*x
distance_threshold: None
settle_duration_ms: 20 #ms
max: 10
min: -10
-
type: SyntheticStage
serial_number: 123
axes: [theta]
axes_mapping: [theta]
max: 360
min: 0
joystick_axes: [x, y, z]
x_max: 100000
x_min: -100000
y_max: 100000
y_min: -100000
z_max: 100000
z_min: -100000
f_max: 100000
f_min: -100000
theta_max: 360
theta_min: 0
x_offset: 0
y_offset: 0
z_offset: 0
theta_offset: 0
f_offset: 0
flip_x: False
flip_y: False
flip_z: False
zoom:
hardware:
type: SyntheticZoom
servo_id: 1
position:
0.63x: 0
1x: 627
2x: 1711
3x: 2301
4x: 2710
5x: 3079
6x: 3383
pixel_size:
0.63x: 9.7
1x: 6.38
2x: 3.14
3x: 2.12
4x: 1.609
5x: 1.255
6x: 1.044
stage_positions:
BABB:
f:
0.63x: 0
1x: 1
2x: 2
3x: 3
4x: 4
5x: 5
6x: 6
shutter:
hardware:
type: SyntheticShutter #NI
channel: PXI6723/port0/line0
min: 0
max: 5
lasers:
- wavelength: 405
onoff:
hardware:
type: NI
channel: PCI6321/port0/line3
min: 0
max: 5
power: #analog
hardware:
type: SyntheticLaser
channel: PCI6321/ao1
min: 0
max: 5
type: LuxX
- wavelength: 488
onoff: #digital
hardware:
type: NI
channel: PCI6321/port0/line5
min: 0
max: 5
power: #analog
hardware:
type: SyntheticLaser
channel: PCI6321/ao0
min: 0
max: 5
type: LuxX
- wavelength: 561
onoff:
hardware:
type: NI
channel: PCI6321/port0/line7
min: 0
max: 5
power: #analog
hardware:
type: SyntheticLaser
channel: PCI6321/ao1
min: 0
max: 5
type: Obis
- wavelength: 640
onoff: #digital
hardware:
type: NI
channel: PCI6321/port0/line1
min: 0
max: 5
power: #analog
hardware:
type: SyntheticLaser
channel: PCI6321/ao2
min: 0
max: 5
type: LuxX
gui:
channels:
count: 5