# Only one microscope can be active in the GUI at a time, but all microscopes will be accessible microscopes: Nanoscale: daq: hardware: type: NI # NI PCIe-1073 Chassis with PXI-6259 and PXI-6733 DAQ Boards. # Sampling rate in Hz sample_rate: 100000 # triggers master_trigger_out_line: PXI6259/port0/line1 camera_trigger_out_line: /PXI6259/ctr0 trigger_source: /PXI6259/PFI0 # Digital Laser Outputs laser_port_switcher: PXI6733/port0/line1 laser_switch_state: False camera: hardware: type: HamamatsuOrcaLightning #SyntheticCamera serial_number: 000035 defect_correct_mode: 2.0 delay: 10 #ms settle_down: 0.0 #ms remote_focus_device: hardware: type: NI channel: PXI6259/ao3 min: -0.5 max: 0.5 # Optotune EL-16-40-TC-VIS-5D-1-C galvo: - hardware: type: NI channel: PXI6259/ao1 min: 0 max: 5 phase: 1.57079 # pi/2 filter_wheel: hardware: type: ASI #SyntheticFilterWheel wheel_number: 2 port: COM8 baudrate: 115200 filter_wheel_delay: .030 # in seconds available_filters: BLU - FF01-442/42-32: 0 GFP - FF01-515/30-32: 1 RFP - FF01-595/31-32: 2 Far-Red - FF01-670/30-32: 3 Blocked1: 4 Empty: 5 Blocked3: 6 Blocked4: 7 Blocked5: 8 Blocked6: 9 stage: hardware: - type: ASI serial_number: 123456789 port: COM8 baudrate: 115200 axes: [x, y, z, f] # Software axes_mapping: [M, Y, X, Z] # M Shear axis mapping #axes_mapping: [M, X, Y, Z] #testing y #axes_mapping: [M, X, Z, Y] #testing Z # axes_mapping: [Z, Y, X, M] feedback_alignment: [90, 90, 90, 90] - name: stage type: SyntheticStage serial_number: 987654321 axes: [theta] x_max: 0 # Swapped from Z x_min: -22708.3 # Swapped from Z y_max: 1361.3 y_min: -3496.3 z_max: 3521.9 # Swapped from X z_min: -4551.1 # Swapped from X f_max: 3233.0 #=m f_min: -9382.0 #=m theta_max: 360 theta_min: 0 external_trigger: /PXI6259/PFI1 # joystick_axes: [x, y, z, f] x_offset: 0 y_offset: 0 z_offset: 0 f_offset: 0 theta_offset: 0 zoom: hardware: type: SyntheticZoom servo_id: 1 position: N/A: 0 pixel_size: N/A: 0.168 shutter: hardware: type: NI channel: PXI6259/port0/line0 min: 0 max: 5 lasers: # Omicron LightHub Ultra # 488 and 640 are LuxX+ Lasers # 561 is a Coherent OBIS Laser # Digital Laser Outputs - wavelength: 405 onoff: hardware: type: NI channel: PXI6733/port0/line2 min: 0 max: 5 power: hardware: type: NI channel: PXI6733/ao0 min: 0 max: 5 type: LuxX - wavelength: 488 onoff: hardware: type: NI channel: PXI6733/port0/line3 min: 0 max: 5 power: hardware: type: NI channel: PXI6733/ao1 min: 0 max: 5 type: LuxX - wavelength: 561 onoff: hardware: type: NI channel: PXI6733/port0/line4 min: 0 max: 5 power: hardware: type: NI channel: PXI6733/ao2 min: 0 max: 5 type: Obis - wavelength: 642 onoff: hardware: type: NI channel: PXI6733/port0/line5 min: 0 max: 5 power: hardware: type: NI channel: PXI6733/ao3 min: 0 max: 5 type: LuxX - wavelength: LED power: hardware: type: NI channel: PXI6733/ao4 min: 0 max: 5 Macroscale: daq: hardware: type: NI # NI PCIe-1073 Chassis with PXI-6259 and PXI-6733 DAQ Boards. # Sampling rate in Hz sample_rate: 100000 # triggers master_trigger_out_line: PXI6259/port0/line1 camera_trigger_out_line: /PXI6259/ctr0 trigger_source: /PXI6259/PFI0 # Digital Laser Outputs laser_port_switcher: PXI6733/port0/line1 laser_switch_state: True camera: hardware: type: Photometrics #SyntheticCamera serial_number: 1 camera_connection: PMPCIECam00 x_pixels: 5056.0 y_pixels: 2960.0 pixel_size_in_microns: 4.25 defect_correct_mode: 2.0 delay: 25 #8 #5.0 settle_down: 0.0 #ms remote_focus_device: hardware: type: NI channel: PXI6259/ao3 min: -0.5 max: 0.5 # Optotune EL-16-40-TC-VIS-5D-1-C galvo: - hardware: type: NI channel: PXI6259/ao1 min: 0 max: 5 phase: 1.57079 # pi/2 filter_wheel: hardware: type: ASI #SyntheticFilterWheel wheel_number: 2 port: COM8 baudrate: 115200 filter_wheel_delay: .030 # in seconds available_filters: BLU - FF01-442/42-32: 0 GFP - FF01-515/30-32: 1 RFP - FF01-595/31-32: 2 Far-Red - FF01-670/30-32: 3 Blocked1: 4 Empty: 5 Blocked3: 6 Blocked4: 7 Blocked5: 8 Blocked6: 9 stage: hardware: - name: stage type: ASI port: COM8 baudrate: 115200 serial_number: 123456789 axes: [x, y, z, f] #Software # axes_mapping: [M, Y, X, Z] axes_mapping: [M, Y, X, Z] #M shear feedback_alignment: [90, 90, 90, 90] - name: stage type: SyntheticStage serial_number: 987654321 axes: [theta] x_max: 0 # Swapped from Z x_min: -22708.3 # Swapped from Z y_max: 1361.3 y_min: -3496.3 z_max: 3521.9 # Swapped from X z_min: -4651.1 # Swapped from X f_max: 3233.0 #=m f_min: -9382.0 #=m theta_max: 360 theta_min: 0 external_trigger: /PXI6259/PFI1 # joystick_axes: [x, y, z, f] x_offset: 0 y_offset: 0 z_offset: 0 f_offset: 0 theta_offset: 0 zoom: hardware: type: SyntheticZoom servo_id: 1 position: N/A: 0 pixel_size: N/A: 1.06 shutter: hardware: type: NI channel: PXI6259/port0/line0 min: 0 max: 5 lasers: # Omicron LightHub Ultra # 488 and 640 are LuxX+ Lasers # 561 is a Coherent OBIS Laser # Digital Laser Outputs - wavelength: 405 onoff: hardware: type: NI channel: PXI6733/port0/line2 min: 0 max: 5 power: hardware: type: NI channel: PXI6733/ao0 min: 0 max: 5 type: LuxX - wavelength: 488 onoff: hardware: type: NI channel: PXI6733/port0/line3 min: 0 max: 5 power: hardware: type: NI channel: PXI6733/ao1 min: 0 max: 5 type: LuxX - wavelength: 561 onoff: hardware: type: NI channel: PXI6733/port0/line4 min: 0 max: 5 power: hardware: type: NI channel: PXI6733/ao2 min: 0 max: 5 type: Obis - wavelength: 642 onoff: hardware: type: NI channel: PXI6733/port0/line5 min: 0 max: 5 power: hardware: type: NI channel: PXI6733/ao3 min: 0 max: 5 type: LuxX - wavelength: LED power: hardware: type: NI channel: PXI6733/ao4 min: 0 max: 5 gui: channels: count: 5