feat: init

test/controller/sub_controllers/test_acquire_bar.py

@@ -0,0 +1,617 @@
+# Copyright (c) 2021-2025  The University of Texas Southwestern Medical Center.
+# All rights reserved.
+import tkinter
+
+# Redistribution and use in source and binary forms, with or without
+# modification, are permitted for academic and research use only
+# (subject to the limitations in the disclaimer below)
+# provided that the following conditions are met:
+
+#      * Redistributions of source code must retain the above copyright notice,
+#      this list of conditions and the following disclaimer.
+
+#      * Redistributions in binary form must reproduce the above copyright
+#      notice, this list of conditions and the following disclaimer in the
+#      documentation and/or other materials provided with the distribution.
+
+#      * Neither the name of the copyright holders nor the names of its
+#      contributors may be used to endorse or promote products derived from this
+#      software without specific prior written permission.
+
+# NO EXPRESS OR IMPLIED LICENSES TO ANY PARTY'S PATENT RIGHTS ARE GRANTED BY
+# THIS LICENSE. THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND
+# CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT
+# LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
+# PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT HOLDER OR
+# CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL,
+# EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO,
+# PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR
+# BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER
+# IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE)
+# ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE
+# POSSIBILITY OF SUCH DAMAGE.
+#
+
+# Standard library imports
+from unittest.mock import MagicMock
+
+# Third party imports
+import pytest
+
+# Local imports
+from navigate.controller.sub_controllers import AcquireBarController
+from navigate.view.popups.acquire_popup import (
+    AcquirePopUp,
+)
+from navigate.model.data_sources import FILE_TYPES
+
+
+class TestAcquireBarController:
+    """Tests for the AcquireBarController class"""
+
+    @pytest.fixture(autouse=True)
+    def setup_class(self, dummy_controller):
+        """Setup for the TestAcquireBarController class
+
+        Parameters
+        ----------
+        dummy_controller : DummyController
+            Instance of the DummyController class
+        """
+        c = dummy_controller
+        v = dummy_controller.view
+
+        self.acquire_bar_controller = AcquireBarController(
+            view=v.acquire_bar, parent_controller=c
+        )
+        self.acquire_bar_controller.populate_experiment_values()
+        c.channels_tab_controller.populate_experiment_values()
+        c.camera_setting_controller = MagicMock()
+
+    def test_init(self):
+        """Tests the initialization of the AcquireBarController class
+
+        Raises
+        ------
+        AssertionError
+            If the initialization of the AcquireBarController class is not correct
+        """
+        assert isinstance(self.acquire_bar_controller, AcquireBarController)
+
+    def test_attr(self):
+        """Tests the attributes of the AcquireBarController class
+
+        Raises
+        ------
+        AssertionError
+            If the attributes of the AcquireBarController class are not correct
+        """
+        # Listing off attributes to check existence
+        attrs = ["mode", "is_save", "mode_dict"]
+
+        for attr in attrs:
+            assert hasattr(self.acquire_bar_controller, attr)
+
+    @pytest.mark.parametrize(
+        "mode,mode_expected,value_expected",
+        [
+            ("live", "indeterminate", None),
+            ("single", "determinate", 0),
+            ("customized", "indeterminate", None),
+            ("z-stack", "determinate", 0),
+        ],
+    )
+    def test_progress_bar(self, mode, mode_expected, value_expected):
+        """Tests the progress bar of the AcquireBarController class
+
+        Parameters
+        ----------
+        mode : str
+            Mode of the progress bar
+        mode_expected : str
+            Expected mode of the progress bar
+        value_expected : int
+            Expected value of the progress bar
+
+
+        Raises
+        ------
+        AssertionError
+            If the progress bar of the AcquireBarController class is not correct
+        """
+
+        # Startup progress bars
+        images_received = 0
+        mode = mode
+        stop = False
+        self.acquire_bar_controller.progress_bar(
+            images_received=images_received,
+            microscope_state=self.acquire_bar_controller.parent_controller.configuration[
+                "experiment"
+            ][
+                "MicroscopeState"
+            ],
+            mode=mode,
+            stop=stop,
+        )
+
+        progress_mode = str(self.acquire_bar_controller.view.CurAcq["mode"])
+        ovr_mode = str(self.acquire_bar_controller.view.OvrAcq["mode"])
+
+        assert progress_mode == mode_expected, (
+            f"Wrong progress bar mode ({progress_mode}) "
+            f"relative to microscope mode ({mode})"
+        )
+        assert ovr_mode == mode_expected, (
+            f"Wrong progress bar mode ({progress_mode}) "
+            f"relative to microscope mode ({mode})"
+        )
+
+        if value_expected is not None:
+            progress_start = int(self.acquire_bar_controller.view.CurAcq["value"])
+            ovr_start = int(self.acquire_bar_controller.view.OvrAcq["value"])
+            assert (
+                progress_start == value_expected
+            ), "Wrong starting value for progress bar"
+            assert ovr_start == value_expected, "Wrong starting value for progress bar"
+
+        # Updating progress bar
+        images_received = 1
+        while images_received < 6:
+            self.acquire_bar_controller.progress_bar(
+                images_received=images_received,
+                microscope_state=self.acquire_bar_controller.parent_controller.configuration[
+                    "experiment"
+                ][
+                    "MicroscopeState"
+                ],
+                mode=mode,
+                stop=stop,
+            )
+            making_progress = float(self.acquire_bar_controller.view.CurAcq["value"])
+            ovr_progress = float(self.acquire_bar_controller.view.OvrAcq["value"])
+            assert (
+                making_progress > 0
+            ), f"Progress bar should be moving in {mode} mode (making_progress)"
+            assert (
+                ovr_progress > 0
+            ), f"Progress bar should be moving in {mode} mode (ovr_progress)"
+            images_received += 1
+
+        # Stopping progress bar
+        self.acquire_bar_controller.progress_bar(
+            images_received=images_received,
+            microscope_state=self.acquire_bar_controller.parent_controller.configuration[
+                "experiment"
+            ][
+                "MicroscopeState"
+            ],
+            mode=mode,
+            stop=True,
+        )
+
+        after_stop = float(self.acquire_bar_controller.view.CurAcq["value"])
+        after_ovr = float(self.acquire_bar_controller.view.OvrAcq["value"])
+
+        assert after_stop == 0, "Progress Bar did not stop"
+        assert after_ovr == 0, "Progress Bar did not stop"
+
+    @pytest.mark.parametrize("mode", ["live", "single", "z-stack", "customized"])
+    def test_get_set_mode(self, mode):
+        """Tests the get_mode and set_mode methods of the AcquireBarController class
+
+        Parameters
+        ----------
+        mode : str
+            Mode of the progress bar
+
+
+        Raises
+        ------
+        AssertionError
+            If the get_mode and set_mode methods of the
+            AcquireBarController class are not correct
+        """
+        self.acquire_bar_controller.set_mode(mode)
+        test = self.acquire_bar_controller.get_mode()
+        assert test == mode, "Mode not set correctly"
+        # assert imaging mode is updated in the experiment
+        assert (
+            self.acquire_bar_controller.parent_controller.configuration["experiment"][
+                "MicroscopeState"
+            ]["image_mode"]
+            == mode
+        )
+
+    def test_set_save(self):
+        """Tests the set_save method of the AcquireBarController class
+
+
+        Raises
+        ------
+        AssertionError
+            If the set_save method of the AcquireBarController class is not correct
+        """
+
+        # Assuming save state starts as False
+        self.acquire_bar_controller.set_save_option(True)
+        assert self.acquire_bar_controller.is_save is True, "Save option not correct"
+
+        # Return value to False
+        self.acquire_bar_controller.set_save_option(False)
+        assert (
+            self.acquire_bar_controller.is_save is False
+        ), "Save option did not return to original value"
+
+    def test_stop_acquire(self):
+        """Tests the stop_acquire method of the AcquireBarController class
+
+
+        Raises
+        ------
+        AssertionError
+            If the stop_acquire method of the AcquireBarController class is not correct
+        """
+
+        # Stopping acquisition
+        self.acquire_bar_controller.stop_acquire()
+        assert self.acquire_bar_controller.view.acquire_btn["text"] == "Acquire"
+
+    @pytest.mark.parametrize(
+        "user_mode,expected_mode",
+        [
+            ("Continuous Scan", "live"),
+            ("Z-Stack", "z-stack"),
+            ("Single Acquisition", "single"),
+            ("Customized", "customized"),
+        ],
+    )
+    def test_update_microscope_mode(self, user_mode, expected_mode):
+        """Tests the update_microscope_mode method of the AcquireBarController class
+
+        Parameters
+        ----------
+        user_mode : str
+            Mode of the progress bar
+        expected_mode : str
+            Expected state of the progress bar
+
+
+        Raises
+        ------
+        AssertionError
+            If the update_microscope_mode method of
+            the AcquireBarController class is not correct
+        """
+        # Assuming mode starts on live
+        self.acquire_bar_controller.mode = "live"
+
+        # Setting to mode specified by user
+        self.acquire_bar_controller.view.pull_down.set(user_mode)
+
+        # Generate event that calls update microscope mode
+        self.acquire_bar_controller.view.pull_down.event_generate(
+            "<<ComboboxSelected>>"
+        )
+
+        # Checking that new mode gets set by function
+        assert self.acquire_bar_controller.mode == expected_mode
+        assert (
+            self.acquire_bar_controller.parent_controller.configuration["experiment"][
+                "MicroscopeState"
+            ]["image_mode"]
+            == expected_mode
+        )
+
+        # Resetting to live
+        self.acquire_bar_controller.view.pull_down.set("Continuous Scan")
+        self.acquire_bar_controller.view.pull_down.event_generate(
+            "<<ComboboxSelected>>"
+        )
+        assert self.acquire_bar_controller.mode == "live"
+
+    def test_populate_experiment_values(self):
+        """Tests the populate_experiment_values method of the AcquireBarController class
+
+
+        Raises
+        ------
+        AssertionError
+            If the populate_experiment_values method
+            of the AcquireBarController class is not correct
+        """
+
+        # Calling function to populate values
+        self.acquire_bar_controller.populate_experiment_values()
+
+        # Checking values are what we expect
+        for key, value in self.acquire_bar_controller.saving_settings.items():
+            assert (
+                self.acquire_bar_controller.saving_settings[key]
+                == self.acquire_bar_controller.parent_controller.configuration[
+                    "experiment"
+                ]["Saving"][key]
+            )
+
+        # Assuming default value in exp file,
+        # can be altered TODO maybe set default to current date
+        assert self.acquire_bar_controller.saving_settings["date"] == "2022-06-07"
+        assert (
+            self.acquire_bar_controller.mode
+            == self.acquire_bar_controller.parent_controller.configuration[
+                "experiment"
+            ]["MicroscopeState"]["image_mode"]
+        )
+
+    @pytest.mark.parametrize(
+        "text,is_acquiring, save,mode,file_types,choice",
+        [
+            ("Stop", False, None, "live", [], None),
+            ("Stop", True, None, "live", [], None),
+            ("Acquire", True, True, "live", [], None),
+            ("Acquire", False, True, "live", [], None),
+            ("Acquire", False, False, "z-stack", [], None),
+            ("Acquire", False, True, "z-stack", FILE_TYPES, "Done"),
+            ("Acquire", False, True, "z-stack", FILE_TYPES, "Cancel"),
+        ],
+    )
+    def test_launch_popup_window(
+        self, text, is_acquiring, save, mode, file_types, choice
+    ):
+        """Tests the launch_popup_window method of the AcquireBarController class
+
+        This is the largest test for this controller.
+        It will test multiple functions that are all used together
+        and difficult to isolate.
+
+        Funcs Tested:
+        launch_popup_window
+        update_file_type
+        launch_acquisition
+        update_experiment_values
+        acquire_pop.popup.dismiss # This will be double tested in view
+
+        Parameters
+        ----------
+        text : str
+            Text of the button that is clicked
+        save : bool
+            Whether or not to save the image
+        mode : str
+            Mode of the progress bar
+        file_types : list
+            List of file types to save as
+        choice : str
+            Choice of the user in the popup window
+
+
+        Raises
+        ------
+        AssertionError
+            If the launch_popup_window method of the
+            AcquireBarController class is not correct
+        """
+
+        # Setup Gui for test
+        self.acquire_bar_controller.view.acquire_btn.configure(state="normal")
+        self.acquire_bar_controller.view.acquire_btn.configure(text=text)
+        self.acquire_bar_controller.is_save = save
+        self.acquire_bar_controller.set_mode(mode)
+        self.acquire_bar_controller.is_acquiring = is_acquiring
+
+        # Test based on setup, launches popup
+        self.acquire_bar_controller.view.acquire_btn.invoke()
+
+        # Checking things are what we expect
+        if text == "Stop":
+            assert self.acquire_bar_controller.view.acquire_btn["text"] == "Stop"
+            if is_acquiring:
+                assert (
+                    str(self.acquire_bar_controller.view.acquire_btn["state"])
+                    == "disabled"
+                )
+                res = self.acquire_bar_controller.parent_controller.pop()
+                assert res == "stop_acquire"
+            else:
+                assert (
+                    str(self.acquire_bar_controller.view.acquire_btn["state"])
+                    == "normal"
+                )
+                res = self.acquire_bar_controller.parent_controller.pop()
+                assert res == "Empty command list"
+
+        if text == "Acquire":
+            if is_acquiring:
+                assert (
+                    str(self.acquire_bar_controller.view.acquire_btn["state"])
+                    == "normal"
+                )
+                res = self.acquire_bar_controller.parent_controller.pop()
+                assert res == "Empty command list"
+                return
+            # First scenario Save is on and in live mode
+            if save is True and mode == "live":
+                assert self.acquire_bar_controller.view.acquire_btn["text"] == "Acquire"
+                assert (
+                    str(self.acquire_bar_controller.view.acquire_btn["state"])
+                    == "disabled"
+                )
+                res = self.acquire_bar_controller.parent_controller.pop()
+                print(res)
+                print(self.acquire_bar_controller.parent_controller.pop())
+                assert res == "acquire"
+
+            # Second scenario Save is off and mode is not live
+            if save is False and mode != "live":
+                assert self.acquire_bar_controller.view.acquire_btn["text"] == "Acquire"
+                assert (
+                    str(self.acquire_bar_controller.view.acquire_btn["state"])
+                    == "disabled"
+                )
+                res = self.acquire_bar_controller.parent_controller.pop()
+                assert res == "acquire"
+
+            # Third and final scenario Save is on and mode is not live
+            if save is True and mode != "live":
+
+                # Checking if popup created
+                assert isinstance(self.acquire_bar_controller.acquire_pop, AcquirePopUp)
+                assert self.acquire_bar_controller.acquire_pop.popup.winfo_exists() == 1
+
+                # Testing update_file_type if list exists
+                widgets = self.acquire_bar_controller.acquire_pop.get_widgets()
+                if len(file_types) > 0:
+                    for file in file_types:
+                        widgets["file_type"].set(file)
+                        assert (
+                            self.acquire_bar_controller.saving_settings["file_type"]
+                            == file
+                        )
+                    # Resetting file type back to orginal
+                    widgets["file_type"].set("TIFF")
+                    assert (
+                        self.acquire_bar_controller.saving_settings["file_type"]
+                        == "TIFF"
+                    )
+
+                # Check that loop thru saving settings is correct
+                for k, v in self.acquire_bar_controller.saving_settings.items():
+                    if widgets.get(k, None):
+                        value = widgets[k].get().strip()
+                        assert value == v
+
+                # Grabbing buttons to test
+                buttons = self.acquire_bar_controller.acquire_pop.get_buttons()
+
+                if choice == "Cancel":
+                    # Testing cancel button
+
+                    buttons["Cancel"].invoke()  # Call to dismiss popup
+                    # Check toplevel gone
+                    assert (
+                        self.acquire_bar_controller.acquire_pop.popup.winfo_exists()
+                        == 0
+                    )
+                    assert (
+                        str(self.acquire_bar_controller.view.acquire_btn["state"])
+                        == "normal"
+                    )
+                elif choice == "Done":
+                    # Testing done button
+
+                    # Update experiment values test
+                    # Changing popup vals to test update
+                    # experiment values inside launch acquisition
+                    widgets["user"].set("John")
+                    widgets["tissue"].set("Heart")
+                    widgets["celltype"].set("34T")
+                    widgets["label"].set("BCB")
+                    widgets["solvent"].set("uDISCO")
+                    widgets["file_type"].set("OME-TIFF")
+
+                    # Tab frame
+                    for i in range(100):
+                        self.acquire_bar_controller.acquire_pop.tab_frame.inputs[
+                            "misc"
+                        ].insert(tkinter.END, f"L{i}")
+
+                    self.acquire_bar_controller.acquire_pop.tab_frame.inputs[
+                        "shear_data"
+                    ].set(True)
+                    self.acquire_bar_controller.acquire_pop.tab_frame.inputs[
+                        "shear_dimension"
+                    ].set("XZ")
+                    self.acquire_bar_controller.acquire_pop.tab_frame.inputs[
+                        "shear_angle"
+                    ].set(45)
+                    self.acquire_bar_controller.acquire_pop.tab_frame.inputs[
+                        "rotate_data"
+                    ].set(True)
+                    self.acquire_bar_controller.acquire_pop.tab_frame.inputs[
+                        "rotate_angle_x"
+                    ].set(90)
+                    self.acquire_bar_controller.acquire_pop.tab_frame.inputs[
+                        "rotate_angle_y"
+                    ].set(90)
+                    self.acquire_bar_controller.acquire_pop.tab_frame.inputs[
+                        "rotate_angle_z"
+                    ].set(90)
+                    self.acquire_bar_controller.acquire_pop.tab_frame.inputs[
+                        "down_sample_data"
+                    ].set(True)
+                    self.acquire_bar_controller.acquire_pop.tab_frame.inputs[
+                        "lateral_down_sample"
+                    ].set("2x")
+                    self.acquire_bar_controller.acquire_pop.tab_frame.inputs[
+                        "axial_down_sample"
+                    ].set("2x")
+
+                    # Launch acquisition start/test
+                    buttons["Done"].invoke()  # Call to launch acquisition
+
+                    # Check if update experiment values works correctly
+                    pop_vals = self.acquire_bar_controller.acquire_pop.get_variables()
+                    for k, v in self.acquire_bar_controller.saving_settings.items():
+                        if pop_vals.get(k, None):
+                            value = pop_vals[k].strip()
+                            assert value == v
+
+                    # Check command sent to controller
+                    # and if acquire button changed to Stop
+                    res = self.acquire_bar_controller.parent_controller.pop()
+                    assert res == "acquire_and_save"
+                    assert (
+                        str(self.acquire_bar_controller.view.acquire_btn["state"])
+                        == "disabled"
+                    )
+                    assert (
+                        self.acquire_bar_controller.acquire_pop.popup.winfo_exists()
+                        == 0
+                    )
+
+    def test_frequent_start_and_stop_acquisition(self):
+        # set up
+        self.acquire_bar_controller.view.acquire_btn.configure(state="normal")
+        self.acquire_bar_controller.view.acquire_btn.configure(text="Acquire")
+        self.acquire_bar_controller.is_save = False
+        self.acquire_bar_controller.set_mode("live")
+        self.acquire_bar_controller.is_acquiring = False
+
+        # start acquisition
+        self.acquire_bar_controller.view.acquire_btn.invoke()
+        assert self.acquire_bar_controller.view.acquire_btn["text"] == "Acquire"
+        assert str(self.acquire_bar_controller.view.acquire_btn["state"]) == "disabled"
+        assert self.acquire_bar_controller.is_acquiring is True
+        # assert dummy_controller_to_test_acquire_bar.acquisition_count == 1
+        res = self.acquire_bar_controller.parent_controller.pop()
+        assert res == "acquire"
+
+        # not in acquisition, click the "Acquire" button several times
+        self.acquire_bar_controller.view.acquire_btn.invoke()
+        assert self.acquire_bar_controller.view.acquire_btn["text"] == "Acquire"
+        assert str(self.acquire_bar_controller.view.acquire_btn["state"]) == "disabled"
+        res = self.acquire_bar_controller.parent_controller.pop()
+        assert res == "Empty command list"
+        self.acquire_bar_controller.view.acquire_btn.invoke()
+        res = self.acquire_bar_controller.parent_controller.pop()
+        assert res == "Empty command list"
+
+        # in acquisition, click "Stop" button several times
+        self.acquire_bar_controller.view.acquire_btn.configure(state="normal")
+        self.acquire_bar_controller.view.acquire_btn.configure(text="Stop")
+        self.acquire_bar_controller.is_acquiring = True
+
+        self.acquire_bar_controller.view.acquire_btn.invoke()
+        assert self.acquire_bar_controller.view.acquire_btn["text"] == "Stop"
+        assert str(self.acquire_bar_controller.view.acquire_btn["state"]) == "disabled"
+        res = self.acquire_bar_controller.parent_controller.pop()
+        assert res == "stop_acquire"
+        self.acquire_bar_controller.view.acquire_btn.invoke()
+        res = self.acquire_bar_controller.parent_controller.pop()
+        assert res == "Empty command list"
+        self.acquire_bar_controller.view.acquire_btn.invoke()
+        res = self.acquire_bar_controller.parent_controller.pop()
+        assert res == "Empty command list"
+        self.acquire_bar_controller.view.acquire_btn.invoke()
+        res = self.acquire_bar_controller.parent_controller.pop()
+        assert res == "Empty command list"

test/controller/sub_controllers/test_autofocus.py

@@ -0,0 +1,202 @@
+# Copyright (c) 2021-2025  The University of Texas Southwestern Medical Center.
+# All rights reserved.
+
+# Redistribution and use in source and binary forms, with or without
+# modification, are permitted for academic and research use only
+# (subject to the limitations in the disclaimer below)
+# provided that the following conditions are met:
+
+#      * Redistributions of source code must retain the above copyright notice,
+#      this list of conditions and the following disclaimer.
+
+#      * Redistributions in binary form must reproduce the above copyright
+#      notice, this list of conditions and the following disclaimer in the
+#      documentation and/or other materials provided with the distribution.
+
+#      * Neither the name of the copyright holders nor the names of its
+#      contributors may be used to endorse or promote products derived from this
+#      software without specific prior written permission.
+
+# NO EXPRESS OR IMPLIED LICENSES TO ANY PARTY'S PATENT RIGHTS ARE GRANTED BY
+# THIS LICENSE. THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND
+# CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT
+# LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
+# PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT HOLDER OR
+# CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL,
+# EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO,
+# PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR
+# BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER
+# IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE)
+# ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE
+# POSSIBILITY OF SUCH DAMAGE.
+#
+
+# Standard library imports
+
+# Third party imports
+import pytest
+import numpy as np
+
+# Local application imports
+from navigate.controller.sub_controllers import AutofocusPopupController
+from navigate.view.popups.autofocus_setting_popup import AutofocusPopup
+
+
+class TestAutofocusPopupController:
+    """Class for testing autofocus popup controller
+
+    Methods
+    -------
+    test_init()
+        Tests that the controller is initialized correctly
+    test_attr()
+        Tests that the attributes are initialized correctly
+    test_populate_experiment_values()
+        Tests that the values are populated correctly
+    test_update_experiment_values()
+        Tests that the values are updated correctly
+    test_start_autofocus()
+        Tests that the start autofocus function works correctly
+    test_display_plot()
+        Tests that the display plot function works correctly
+    """
+
+    @pytest.fixture(autouse=True)
+    def setup_class(self, dummy_controller):
+        """Setup for testing autofocus popup controller
+
+        Parameters
+        ----------
+        dummy_controller : DummyController
+            Dummy controller for testing
+
+        """
+        autofocus_popup = AutofocusPopup(dummy_controller.view)
+        self.autofocus_controller = AutofocusPopupController(
+            autofocus_popup, dummy_controller
+        )
+
+    def test_init(self):
+        """Tests that the controller is initialized correctly
+
+        Raises
+        ------
+        AssertionError
+            If the controller is not initialized correctly
+        """
+        assert isinstance(self.autofocus_controller, AutofocusPopupController)
+        assert self.autofocus_controller.view.popup.winfo_exists() == 1
+
+    def test_attr(self):
+        """Tests that the attributes are initialized correctly
+
+        Raises
+        ------
+        AssertionError
+            If the attributes are not initialized correctly
+        """
+
+        # Listing off attributes to check existence
+        attrs = [
+            "autofocus_fig",
+            "autofocus_coarse",
+            "widgets",
+            "setting_dict",
+        ]
+
+        for attr in attrs:
+            assert hasattr(self.autofocus_controller, attr)
+
+    def test_populate_experiment_values(self):
+        """Tests that the values are populated correctly
+
+        Raises
+        ------
+        AssertionError
+            If the values are not populated correctly
+        """
+        microscope_name = self.autofocus_controller.microscope_name
+        device = self.autofocus_controller.widgets["device"].get()
+        device_ref = self.autofocus_controller.widgets["device_ref"].get()
+        for k in self.autofocus_controller.widgets:
+            if k != "device" and k != "device_ref":
+                assert self.autofocus_controller.widgets[k].get() == str(
+                    self.autofocus_controller.setting_dict[microscope_name][device][
+                        device_ref
+                    ][k]
+                )
+            # Some values are ints but Tkinter only uses strings
+
+    def test_update_experiment_values(self):
+        """Tests that the values are updated correctly
+
+        Raises
+        ------
+        AssertionError
+            If the values are not updated correctly
+        """
+        # Changing values
+        self.autofocus_controller.widgets["coarse_range"].set(200)
+        self.autofocus_controller.widgets["coarse_step_size"].set(30)
+        self.autofocus_controller.view.setting_vars["coarse_selected"].set(False)
+        self.autofocus_controller.widgets["fine_range"].set(25)
+        self.autofocus_controller.widgets["fine_step_size"].set(2)
+        self.autofocus_controller.view.setting_vars["fine_selected"].set(False)
+
+        microscope_name = self.autofocus_controller.microscope_name
+        device = self.autofocus_controller.widgets["device"].get()
+        device_ref = self.autofocus_controller.widgets["device_ref"].get()
+
+        # Checking values match
+        for k in self.autofocus_controller.widgets:
+            if k != "device" and k != "device_ref":
+                assert self.autofocus_controller.widgets[k].get() == str(
+                    self.autofocus_controller.setting_dict[microscope_name][device][
+                        device_ref
+                    ][k]
+                )
+        for k in self.autofocus_controller.view.setting_vars:
+            assert (
+                self.autofocus_controller.view.setting_vars[k].get()
+                == self.autofocus_controller.setting_dict[microscope_name][device][
+                    device_ref
+                ][k]
+            )
+
+    def test_start_autofocus(self):
+        """Tests that the start autofocus function works correctly
+
+        Raises
+        ------
+        AssertionError
+            If the start autofocus function does not work correctly
+        """
+
+        # Calling function
+        self.autofocus_controller.start_autofocus()
+
+        # Checking message sent
+        res = self.autofocus_controller.parent_controller.pop()
+        assert res == "autofocus"
+
+        self.autofocus_controller.parent_controller.clear()
+
+    def test_display_plot(self):
+        """Tests that the display plot function works correctly
+
+        Todo: Retrieve data from axessubplot instance and
+        check that it is correct
+
+        Raises
+        ------
+        AssertionError
+            If the display plot function does not work correctly
+        """
+        # Make this robust by sending data and then
+        # checking each plot is plotting correct data low priority
+
+        x_data = np.linspace(start=69750.0, stop=70250.0, num=101)
+        y_data = np.random.rand(101)
+        data = [x_data, y_data]
+        self.autofocus_controller.display_plot([data, False, True])
+        pass

test/controller/sub_controllers/test_camera_settings.py

@@ -0,0 +1,607 @@
+# Copyright (c) 2021-2025  The University of Texas Southwestern Medical Center.
+# All rights reserved.
+
+# Redistribution and use in source and binary forms, with or without
+# modification, are permitted for academic and research use only
+# (subject to the limitations in the disclaimer below)
+# provided that the following conditions are met:
+
+#      * Redistributions of source code must retain the above copyright notice,
+#      this list of conditions and the following disclaimer.
+
+#      * Redistributions in binary form must reproduce the above copyright
+#      notice, this list of conditions and the following disclaimer in the
+#      documentation and/or other materials provided with the distribution.
+
+#      * Neither the name of the copyright holders nor the names of its
+#      contributors may be used to endorse or promote products derived from this
+#      software without specific prior written permission.
+
+# NO EXPRESS OR IMPLIED LICENSES TO ANY PARTY'S PATENT RIGHTS ARE GRANTED BY
+# THIS LICENSE. THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND
+# CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT
+# LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
+# PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT HOLDER OR
+# CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL,
+# EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO,
+# PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR
+# BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER
+# IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE)
+# ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE
+# POSSIBILITY OF SUCH DAMAGE.
+#
+
+# Standard Imports
+
+# Third Party Imports
+import pytest
+import random
+
+# Local Imports
+from navigate.controller.sub_controllers.camera_settings import (
+    CameraSettingController,
+)
+
+
+class TestCameraSettingController:
+    @pytest.fixture(autouse=True)
+    def setup_class(self, dummy_controller):
+        c = dummy_controller
+        v = dummy_controller.view
+        self.camera_settings = CameraSettingController(
+            v.settings.camera_settings_tab, c
+        )
+
+    def test_init(self):
+
+        assert isinstance(self.camera_settings, CameraSettingController)
+
+        # Setup, going to check what the default values widgets are set too
+        microscope_name = self.camera_settings.parent_controller.configuration[
+            "experiment"
+        ]["MicroscopeState"]["microscope_name"]
+        camera_config_dict = self.camera_settings.parent_controller.configuration[
+            "configuration"
+        ]["microscopes"][microscope_name]["camera"]
+
+        # Default Values
+        assert (
+            self.camera_settings.default_pixel_size
+            == camera_config_dict["pixel_size_in_microns"]
+        )
+        assert self.camera_settings.default_height == camera_config_dict["y_pixels"]
+        assert self.camera_settings.default_width == camera_config_dict["x_pixels"]
+
+        # Camera Mode
+        assert list(self.camera_settings.mode_widgets["Sensor"].widget["values"]) == [
+            "Normal",
+            "Light-Sheet",
+        ]
+        assert (
+            str(self.camera_settings.mode_widgets["Sensor"].widget["state"])
+            == "readonly"
+        )
+
+        # Readout Mode
+        assert list(self.camera_settings.mode_widgets["Readout"].widget["values"]) == [
+            "Top-to-Bottom",
+            "Bottom-to-Top",
+            "Bidirectional",
+            "Rev. Bidirectional",
+        ]
+        assert (
+            str(self.camera_settings.mode_widgets["Readout"].widget["state"])
+            == "disabled"
+        )
+
+        # Pixels
+        assert (
+            str(self.camera_settings.mode_widgets["Pixels"].widget["state"])
+            == "disabled"
+        )
+        assert self.camera_settings.mode_widgets["Pixels"].widget.get() == ""
+        assert self.camera_settings.mode_widgets["Pixels"].widget.cget("from") == 1
+        assert (
+            self.camera_settings.mode_widgets["Pixels"].widget.cget("to")
+            == self.camera_settings.default_height / 2
+        )
+        assert self.camera_settings.mode_widgets["Pixels"].widget.cget("increment") == 1
+
+        # Framerate
+        assert (
+            str(self.camera_settings.framerate_widgets["exposure_time"].widget["state"])
+            == "disabled"
+        )
+        assert (
+            str(self.camera_settings.framerate_widgets["readout_time"].widget["state"])
+            == "disabled"
+        )
+        assert (
+            str(self.camera_settings.framerate_widgets["max_framerate"].widget["state"])
+            == "disabled"
+        )
+
+        # Set range value
+        assert (
+            self.camera_settings.roi_widgets["Width"].widget.cget("to")
+            == self.camera_settings.default_width
+        )
+        assert self.camera_settings.roi_widgets["Width"].widget.cget("from") == 2
+        assert self.camera_settings.roi_widgets["Width"].widget.cget("increment") == 2
+        assert (
+            self.camera_settings.roi_widgets["Height"].widget.cget("to")
+            == self.camera_settings.default_height
+        )
+        assert self.camera_settings.roi_widgets["Height"].widget.cget("from") == 2
+        assert self.camera_settings.roi_widgets["Height"].widget.cget("increment") == 2
+
+        # Set binning options
+        assert list(self.camera_settings.roi_widgets["Binning"].widget["values"]) == [
+            "{}x{}".format(i, i) for i in [1, 2, 4]
+        ]
+        assert (
+            str(self.camera_settings.roi_widgets["Binning"].widget["state"])
+            == "readonly"
+        )
+
+        # FOV
+        assert (
+            str(self.camera_settings.roi_widgets["FOV_X"].widget["state"]) == "disabled"
+        )
+        assert (
+            str(self.camera_settings.roi_widgets["FOV_Y"].widget["state"]) == "disabled"
+        )
+
+    def test_attr(self):
+
+        attrs = [
+            "in_initialization",
+            "resolution_value",
+            "mode",
+            "solvent",
+            "mode_widgets",
+            "framerate_widgets",
+            "roi_widgets",
+            "roi_btns",
+            "default_pixel_size",
+            "default_width",
+            "default_height",
+            "pixel_event_id",
+        ]
+
+        for attr in attrs:
+            assert hasattr(self.camera_settings, attr)
+
+    def test_populate_experiment_values(self):
+        microscope_name = self.camera_settings.parent_controller.configuration[
+            "experiment"
+        ]["MicroscopeState"]["microscope_name"]
+        self.camera_settings.parent_controller.configuration["experiment"][
+            "CameraParameters"
+        ][microscope_name]["readout_time"] = 0.1
+        # Populate widgets with values from experiment file and check
+        self.camera_settings.populate_experiment_values()
+        camera_setting_dict = self.camera_settings.parent_controller.configuration[
+            "experiment"
+        ]["CameraParameters"][microscope_name]
+
+        # Checking values altered are correct
+        assert dict(self.camera_settings.camera_setting_dict) == dict(
+            self.camera_settings.parent_controller.configuration["experiment"][
+                "CameraParameters"
+            ][microscope_name]
+        )
+        assert (
+            str(self.camera_settings.mode_widgets["Sensor"].get())
+            == camera_setting_dict["sensor_mode"]
+        )
+        if camera_setting_dict["sensor_mode"] == "Normal":
+            pass
+            # assert str(self.camera_settings.mode_widgets[
+            #                "Readout"].get()) == ""
+            # assert str(self.camera_settings.mode_widgets[
+            #                "Pixels"].get()) == ""
+        elif camera_setting_dict["sensor_mode"] == "Light-Sheet":
+            assert (
+                str(self.camera_settings.mode_widgets["Readout"].get())
+                == self.camera_settings.camera_setting_dict["readout_direction"]
+            )
+            assert (
+                str(self.camera_settings.mode_widgets["Pixels"].get())
+                == self.camera_settings.camera_setting_dict["number_of_pixels"]
+            )
+
+        # ROI
+        assert (
+            self.camera_settings.roi_widgets["Width"].get()
+            == camera_setting_dict["x_pixels"]
+        )
+        assert (
+            self.camera_settings.roi_widgets["Height"].get()
+            == camera_setting_dict["y_pixels"]
+        )
+
+        assert self.camera_settings.roi_widgets[
+            "Top_X"
+        ].get() == camera_setting_dict.get("top_x", 0)
+        assert self.camera_settings.roi_widgets[
+            "Top_Y"
+        ].get() == camera_setting_dict.get("top_y", 0)
+        if camera_setting_dict.get("is_centered", True):
+            assert (
+                str(self.camera_settings.roi_widgets["Top_X"].widget["state"])
+                == "disabled"
+            )
+            assert (
+                str(self.camera_settings.roi_widgets["Top_Y"].widget["state"])
+                == "disabled"
+            )
+
+        # Binning
+        assert (
+            str(self.camera_settings.roi_widgets["Binning"].get())
+            == camera_setting_dict["binning"]
+        )
+
+        # Exposure Time
+        channels = self.camera_settings.parent_controller.configuration["experiment"][
+            "MicroscopeState"
+        ]["channels"]
+        exposure_time = channels[list(channels.keys())[0]]["camera_exposure_time"]
+        assert (
+            self.camera_settings.framerate_widgets["exposure_time"].get()
+            == exposure_time
+        )
+        assert (
+            self.camera_settings.framerate_widgets["frames_to_average"].get()
+            == camera_setting_dict["frames_to_average"]
+        )
+        assert self.camera_settings.in_initialization is False
+
+    @pytest.mark.parametrize("mode", ["Normal", "Light-Sheet"])
+    def test_update_experiment_values(self, mode):
+
+        microscope_name = self.camera_settings.parent_controller.configuration[
+            "experiment"
+        ]["MicroscopeState"]["microscope_name"]
+        # Setup basic default experiment
+        self.camera_settings.camera_setting_dict = (
+            self.camera_settings.parent_controller.configuration["experiment"][
+                "CameraParameters"
+            ][microscope_name]
+        )
+
+        # Setting up new values in widgets
+        self.camera_settings.mode_widgets["Sensor"].set(mode)
+        self.camera_settings.roi_widgets["Binning"].set("4x4")
+        if mode == "Light-Sheet":
+            self.camera_settings.mode_widgets["Readout"].set("Bottom-to-Top")
+            self.camera_settings.mode_widgets["Pixels"].set(15)
+            self.camera_settings.roi_widgets["Binning"].set("1x1")
+        width, height = random.randint(1, 2000), random.randint(1, 2000)
+        self.camera_settings.roi_widgets["Width"].set(width)
+        self.camera_settings.roi_widgets["Height"].set(height)
+        self.camera_settings.framerate_widgets["frames_to_average"].set(5)
+
+        # Update experiment dict and assert
+        self.camera_settings.update_experiment_values()
+        assert self.camera_settings.camera_setting_dict["sensor_mode"] == mode
+        if mode == "Light-Sheet":
+            assert (
+                self.camera_settings.camera_setting_dict["readout_direction"]
+                == "Bottom-to-Top"
+            )
+            assert (
+                int(self.camera_settings.camera_setting_dict["number_of_pixels"]) == 15
+            )
+        step_width = self.camera_settings.step_width
+        step_height = self.camera_settings.step_height
+        set_width = int(width // step_width) * step_width
+        set_height = int(height // step_height) * step_height
+        if mode == "Light-Sheet":
+            assert self.camera_settings.camera_setting_dict["binning"] == "1x1"
+            assert self.camera_settings.camera_setting_dict["img_x_pixels"] == set_width
+            assert (
+                self.camera_settings.camera_setting_dict["img_y_pixels"] == set_height
+            )
+            binning = 1
+        else:
+            assert self.camera_settings.camera_setting_dict["binning"] == "4x4"
+            # make sure image size is divisible by step_width and step_height
+            assert self.camera_settings.camera_setting_dict["img_x_pixels"] == (
+                set_width // 4
+            ) - (set_width // 4 % step_width)
+            assert self.camera_settings.camera_setting_dict["img_y_pixels"] == (
+                set_height // 4
+            ) - (set_height // 4 % step_height)
+            binning = 4
+
+        # make sure x, y pixels are img_x, img_y pixels * binning
+        assert (
+            self.camera_settings.camera_setting_dict["x_pixels"]
+            == self.camera_settings.camera_setting_dict["img_x_pixels"] * binning
+        )
+        assert (
+            self.camera_settings.camera_setting_dict["y_pixels"]
+            == self.camera_settings.camera_setting_dict["img_y_pixels"] * binning
+        )
+        assert (
+            self.camera_settings.camera_setting_dict["pixel_size"]
+            == self.camera_settings.default_pixel_size
+        )
+        assert self.camera_settings.camera_setting_dict["frames_to_average"] == 5
+
+    @pytest.mark.parametrize("mode", ["Normal", "Light-Sheet"])
+    def test_update_sensor_mode(self, mode):
+        self.camera_settings.populate_experiment_values()
+        microscope_name = self.camera_settings.parent_controller.configuration[
+            "experiment"
+        ]["MicroscopeState"]["microscope_name"]
+        camera_setting_dict = self.camera_settings.parent_controller.configuration[
+            "experiment"
+        ]["CameraParameters"][microscope_name]
+
+        # Set mode
+        self.camera_settings.mode_widgets["Sensor"].widget.set(mode)
+        self.camera_settings.mode_widgets["Sensor"].widget.event_generate(
+            "<<ComboboxSelected>>"
+        )
+
+        # Call update
+        # self.camera_settings.update_sensor_mode()
+
+        # Check values
+        if mode == "Normal":
+            assert str(self.camera_settings.mode_widgets["Readout"].get()) == " "
+            assert (
+                str(self.camera_settings.mode_widgets["Readout"].widget["state"])
+                == "disabled"
+            )
+            assert (
+                str(self.camera_settings.mode_widgets["Pixels"].widget["state"])
+                == "disabled"
+            )
+            assert str(self.camera_settings.mode_widgets["Pixels"].widget.get()) == ""
+
+        if mode == "Light-Sheet":
+            assert (
+                str(self.camera_settings.mode_widgets["Readout"].get())
+                == camera_setting_dict["readout_direction"]
+            )
+            assert (
+                str(self.camera_settings.mode_widgets["Readout"].widget["state"])
+                == "readonly"
+            )
+            assert (
+                str(self.camera_settings.mode_widgets["Pixels"].widget["state"])
+                == "normal"
+            )
+            assert int(self.camera_settings.mode_widgets["Pixels"].widget.get()) == int(
+                self.camera_settings.camera_setting_dict["number_of_pixels"]
+            )
+
+    def test_update_exposure_time(self):
+
+        # Call funciton
+        self.camera_settings.update_exposure_time(35)
+
+        # Check
+        assert self.camera_settings.framerate_widgets["exposure_time"].get() == 35
+
+    @pytest.mark.parametrize("name", ["All", "1600", "1024", "512"])
+    def test_update_roi(self, name):
+
+        # Call button to check if handler setup correctly
+        self.camera_settings.roi_btns[name].invoke()
+
+        # Check
+        if name == "All":
+            name = "2048"
+        assert str(self.camera_settings.roi_widgets["Width"].get()) == name
+        assert str(self.camera_settings.roi_widgets["Height"].get()) == name
+
+    def test_update_fov(self):
+        self.camera_settings.populate_experiment_values()
+
+        # Change invoke
+        self.camera_settings.in_initialization = False
+        self.camera_settings.roi_widgets["Width"].widget.set(2048)
+        self.camera_settings.roi_widgets["Height"].widget.set(2048)
+        xFov = int(self.camera_settings.roi_widgets["FOV_X"].get())
+        yFov = int(self.camera_settings.roi_widgets["FOV_Y"].get())
+        self.camera_settings.roi_widgets["Width"].widget.set(1600)
+        self.camera_settings.roi_widgets["Height"].widget.set(1600)
+
+        # need these since we switched to read events
+        self.camera_settings.roi_widgets["Width"].get_variable().get()
+        self.camera_settings.roi_widgets["Height"].get_variable().get()
+
+        # Check
+        assert xFov != int(self.camera_settings.roi_widgets["FOV_X"].get())
+        assert yFov != int(self.camera_settings.roi_widgets["FOV_Y"].get())
+
+        # Reset
+        self.camera_settings.roi_widgets["Width"].widget.set(2048)
+        self.camera_settings.roi_widgets["Height"].widget.set(2048)
+
+        # need these since we switched to read events
+        self.camera_settings.roi_widgets["Width"].get_variable().get()
+        self.camera_settings.roi_widgets["Height"].get_variable().get()
+
+        assert int(self.camera_settings.roi_widgets["FOV_X"].get()) == 13066
+        assert int(self.camera_settings.roi_widgets["FOV_Y"].get()) == 13066
+
+    @pytest.mark.parametrize("mode", ["live", "z-stack", "stop", "single"])
+    @pytest.mark.parametrize("readout", ["Normal", "Light-Sheet"])
+    def test_set_mode(self, mode, readout):
+
+        # Populate widgets with values from experiment file
+        self.camera_settings.populate_experiment_values()
+
+        # Set mode
+        self.camera_settings.mode_widgets["Sensor"].widget.set(readout)
+        self.camera_settings.update_sensor_mode()
+        self.camera_settings.set_mode(mode)
+
+        # Check
+        assert self.camera_settings.mode == mode
+        if mode != "stop":
+            state = "disabled"
+        else:
+            state = "normal"
+        if mode != "stop":
+            state_readonly = "disabled"
+        else:
+            state_readonly = "readonly"
+
+        assert (
+            str(self.camera_settings.mode_widgets["Sensor"].widget["state"])
+            == state_readonly
+        )
+        if str(self.camera_settings.mode_widgets["Sensor"].get()) == "Light-Sheet":
+            assert (
+                str(self.camera_settings.mode_widgets["Readout"].widget["state"])
+                == state_readonly
+            )
+            if mode == "live":
+                assert (
+                    str(self.camera_settings.mode_widgets["Pixels"].widget["state"])
+                    == "normal"
+                )
+            else:
+                assert (
+                    str(self.camera_settings.mode_widgets["Pixels"].widget["state"])
+                    == state
+                )
+        else:
+            assert (
+                str(self.camera_settings.mode_widgets["Readout"].widget["state"])
+                == "disabled"
+            )
+            assert (
+                str(self.camera_settings.mode_widgets["Pixels"].widget["state"])
+                == "disabled"
+            )
+        assert (
+            str(
+                self.camera_settings.framerate_widgets["frames_to_average"].widget[
+                    "state"
+                ]
+            )
+            == state
+        )
+        assert str(self.camera_settings.roi_widgets["Width"].widget["state"]) == state
+        assert str(self.camera_settings.roi_widgets["Height"].widget["state"]) == state
+        assert (
+            str(self.camera_settings.roi_widgets["Binning"].widget["state"])
+            == state_readonly
+        )
+        for btn_name in self.camera_settings.roi_btns:
+            assert str(self.camera_settings.roi_btns[btn_name]["state"]) == state
+
+    @pytest.mark.parametrize("zoom", ["0.63x", "1x", "2x", "3x", "4x", "5x", "6x"])
+    def test_calculate_physical_dimensions(self, zoom):
+        self.camera_settings.parent_controller.configuration["experiment"][
+            "MicroscopeState"
+        ]["zoom"] = zoom
+
+        self.camera_settings.populate_experiment_values()
+
+        # Calling
+        self.camera_settings.calculate_physical_dimensions()
+
+        pixel_size = self.camera_settings.default_pixel_size
+        x_pixel = float(self.camera_settings.roi_widgets["Width"].get())
+        y_pixel = float(self.camera_settings.roi_widgets["Height"].get())
+
+        microscope_state_dict = self.camera_settings.parent_controller.configuration[
+            "experiment"
+        ]["MicroscopeState"]
+        zoom = microscope_state_dict["zoom"]
+        microscope_name = microscope_state_dict["microscope_name"]
+        pixel_size = self.camera_settings.parent_controller.configuration[
+            "configuration"
+        ]["microscopes"][microscope_name]["zoom"]["pixel_size"][zoom]
+
+        dim_x = x_pixel * pixel_size
+        dim_y = y_pixel * pixel_size
+
+        assert float(self.camera_settings.roi_widgets["FOV_X"].get()) == float(
+            int(dim_x)
+        )
+        assert float(self.camera_settings.roi_widgets["FOV_Y"].get()) == float(
+            int(dim_y)
+        )
+
+        # Reset to zoom of 1
+        self.camera_settings.parent_controller.configuration["experiment"][
+            "MicroscopeState"
+        ]["zoom"] = "1x"
+        assert (
+            self.camera_settings.parent_controller.configuration["experiment"][
+                "MicroscopeState"
+            ]["zoom"]
+            == "1x"
+        )
+
+    def test_calculate_readout_time(self):
+        """
+        TODO need more info about camera before testing
+        """
+        pass
+
+    @pytest.mark.parametrize(
+        "mode", ["single", "live", "customized", "z-stack", "stop"]
+    )
+    def test_update_number_of_pixels(self, mode):
+        import random
+
+        self.camera_settings.populate_experiment_values()
+
+        self.camera_settings.mode = mode
+
+        self.camera_settings.mode_widgets["Pixels"].set("")
+        assert self.camera_settings.camera_setting_dict["number_of_pixels"] != ""
+
+        n_pixels = random.randint(1, 100)
+        self.camera_settings.mode_widgets["Pixels"].set(n_pixels)
+
+        # Check
+        assert self.camera_settings.camera_setting_dict["number_of_pixels"] == int(
+            n_pixels
+        )
+        if mode != "live" and mode != "stop":
+            assert (
+                self.camera_settings.camera_setting_dict["number_of_pixels"] == n_pixels
+            )
+
+    @pytest.mark.parametrize(
+        "x_pixels, y_pixels", [(512, 512), (4096, 4096), (2304, 1024), (1024, 2048)]
+    )
+    def test_update_camera_device_related_setting(self, x_pixels, y_pixels):
+        self.camera_settings.populate_experiment_values()
+
+        microscope_name = self.camera_settings.parent_controller.configuration[
+            "experiment"
+        ]["MicroscopeState"]["microscope_name"]
+        camera_config = self.camera_settings.parent_controller.configuration[
+            "configuration"
+        ]["microscopes"][microscope_name]["camera"]
+        default_x_pixels = camera_config["x_pixels"]
+        default_y_pixels = camera_config["y_pixels"]
+
+        camera_config["x_pixels"] = x_pixels
+        camera_config["y_pixels"] = y_pixels
+        self.camera_settings.update_camera_device_related_setting()
+
+        assert self.camera_settings.roi_widgets["Width"].get() == min(
+            self.camera_settings.camera_setting_dict["x_pixels"], x_pixels
+        )
+        assert self.camera_settings.roi_widgets["Height"].get() == min(
+            self.camera_settings.camera_setting_dict["y_pixels"], y_pixels
+        )
+        assert self.camera_settings.roi_widgets["Width"].widget["to"] == x_pixels
+        assert self.camera_settings.roi_widgets["Height"].widget["to"] == y_pixels
+
+        camera_config["x_pixels"] = default_x_pixels
+        camera_config["y_pixels"] = default_y_pixels

test/controller/sub_controllers/test_camera_view.py

@@ -0,0 +1,813 @@
+# Copyright (c) 2021-2025  The University of Texas Southwestern Medical Center.
+# All rights reserved.
+
+# Redistribution and use in source and binary forms, with or without
+# modification, are permitted for academic and research use only
+# (subject to the limitations in the disclaimer below)
+# provided that the following conditions are met:
+
+#      * Redistributions of source code must retain the above copyright notice,
+#      this list of conditions and the following disclaimer.
+
+#      * Redistributions in binary form must reproduce the above copyright
+#      notice, this list of conditions and the following disclaimer in the
+#      documentation and/or other materials provided with the distribution.
+
+#      * Neither the name of the copyright holders nor the names of its
+#      contributors may be used to endorse or promote products derived from this
+#      software without specific prior written permission.
+
+# NO EXPRESS OR IMPLIED LICENSES TO ANY PARTY'S PATENT RIGHTS ARE GRANTED BY
+# THIS LICENSE. THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND
+# CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT
+# LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
+# PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT HOLDER OR
+# CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL,
+# EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO,
+# PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR
+# BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER
+# IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE)
+# ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE
+# POSSIBILITY OF SUCH DAMAGE.
+#
+
+from navigate.controller.sub_controllers.camera_view import CameraViewController
+import pytest
+import random
+from unittest.mock import MagicMock
+import numpy as np
+
+
+class TestCameraViewController:
+    @pytest.fixture(autouse=True)
+    def setup_class(self, dummy_controller):
+        c = dummy_controller
+        self.v = dummy_controller.view
+        c.model = MagicMock()
+        c.model.get_offset_variance_maps = MagicMock(return_value=[None, None])
+
+        self.camera_view = CameraViewController(self.v.camera_waveform.camera_tab, c)
+
+        self.microscope_state = {
+            "channels": {
+                "channel_1": {
+                    "is_selected": True,
+                    "laser": "488nm",
+                    "laser_index": 0,
+                    "camera_exposure_time": 200.0,
+                    "laser_power": 20.0,
+                    "interval_time": 1.0,
+                    "defocus": 100.0,
+                    "filter_wheel_0": "Empty-Alignment",
+                    "filter_position_0": 0,
+                    "filter_wheel_1": "Empty-Alignment",
+                    "filter_position_1": 0,
+                },
+                "channel_2": {
+                    "is_selected": True,
+                    "laser": "488nm",
+                    "laser_index": 0,
+                    "camera_exposure_time": 200.0,
+                    "laser_power": 20.0,
+                    "interval_time": 1.0,
+                    "defocus": 100.0,
+                    "filter_wheel_0": "Empty-Alignment",
+                    "filter_position_0": 0,
+                    "filter_wheel_1": "Empty-Alignment",
+                    "filter_position_1": 0,
+                },
+                "channel_3": {
+                    "is_selected": True,
+                    "laser": "488nm",
+                    "laser_index": 0,
+                    "camera_exposure_time": 200.0,
+                    "laser_power": 20.0,
+                    "interval_time": 1.0,
+                    "defocus": 100.0,
+                    "filter_wheel_0": "Empty-Alignment",
+                    "filter_position_0": 0,
+                    "filter_wheel_1": "Empty-Alignment",
+                    "filter_position_1": 0,
+                },
+            },
+            "number_z_steps": np.random.randint(10, 100),
+            "stack_cycling_mode": "per_stack",
+            "image_mode": "z-stack",
+        }
+
+    def test_init(self):
+
+        assert isinstance(self.camera_view, CameraViewController)
+
+    def test_update_display_state(self):
+        pass
+
+    def test_get_absolute_position(self, monkeypatch):
+        def mock_winfo_pointerx():
+            self.x = int(random.random())
+            return self.x
+
+        def mock_winfo_pointery():
+            self.y = int(random.random())
+            return self.y
+
+        monkeypatch.setattr(self.v, "winfo_pointerx", mock_winfo_pointerx)
+        monkeypatch.setattr(self.v, "winfo_pointery", mock_winfo_pointery)
+
+        # call the function under test
+        x, y = self.camera_view.get_absolute_position()
+
+        # make assertions about the return value
+        assert x == self.x
+        assert y == self.y
+
+    def test_popup_menu(self, monkeypatch):
+
+        # create a fake event object
+        self.startx = int(random.random())
+        self.starty = int(random.random())
+        event = type("Event", (object,), {"x": self.startx, "y": self.starty})()
+        self.grab_released = False
+        self.x = int(random.random())
+        self.y = int(random.random())
+        self.absx = int(random.random())
+        self.absy = int(random.random())
+
+        # monkey patch the get_absolute_position method to return specific values
+        def mock_get_absolute_position():
+            self.absx = int(random.random())
+            self.absy = int(random.random())
+            return self.absx, self.absy
+
+        monkeypatch.setattr(
+            self.camera_view, "get_absolute_position", mock_get_absolute_position
+        )
+
+        def mock_tk_popup(x, y):
+            self.x = x
+            self.y = y
+
+        def mock_grab_release():
+            self.grab_released = True
+
+        monkeypatch.setattr(self.camera_view.menu, "tk_popup", mock_tk_popup)
+        monkeypatch.setattr(self.camera_view.menu, "grab_release", mock_grab_release)
+
+        # call the function under test
+        self.camera_view.popup_menu(event)
+
+        # make assertions about the state of the view object
+        assert self.camera_view.move_to_x == self.startx
+        assert self.camera_view.move_to_y == self.starty
+        assert self.x == self.absx
+        assert self.y == self.absy
+        assert self.grab_released is True
+
+    @pytest.mark.parametrize("name", ["minmax", "image"])
+    @pytest.mark.parametrize("data", [[random.randint(0, 49), random.randint(50, 100)]])
+    def test_initialize(self, name, data):
+
+        self.camera_view.initialize(name, data)
+
+        # Checking values
+        if name == "minmax":
+            assert self.camera_view.image_palette["Min"].get() == data[0]
+            assert self.camera_view.image_palette["Max"].get() == data[1]
+        if name == "image":
+            assert self.camera_view.image_metrics["Frames"].get() == data[0]
+
+    def test_set_mode(self):
+
+        # Test default mode
+        self.camera_view.set_mode()
+        assert self.camera_view.mode == ""
+        assert self.camera_view.menu.entrycget("Move Here", "state") == "disabled"
+
+        # Test 'live' mode
+        self.camera_view.set_mode("live")
+        assert self.camera_view.mode == "live"
+        assert self.camera_view.menu.entrycget("Move Here", "state") == "normal"
+
+        # Test 'stop' mode
+        self.camera_view.set_mode("stop")
+        assert self.camera_view.mode == "stop"
+        assert self.camera_view.menu.entrycget("Move Here", "state") == "normal"
+
+        # Test invalid mode
+        self.camera_view.set_mode("invalid")
+        assert self.camera_view.mode == "invalid"
+        assert self.camera_view.menu.entrycget("Move Here", "state") == "disabled"
+
+    @pytest.mark.parametrize("mode", ["stop", "live"])
+    def test_move_stage(self, mode):
+
+        # Setup to check formula inside func is correct
+        microscope_name = self.camera_view.parent_controller.configuration[
+            "experiment"
+        ]["MicroscopeState"]["microscope_name"]
+        zoom_value = self.camera_view.parent_controller.configuration["experiment"][
+            "MicroscopeState"
+        ]["zoom"]
+        pixel_size = self.camera_view.parent_controller.configuration["configuration"][
+            "microscopes"
+        ][microscope_name]["zoom"]["pixel_size"][zoom_value]
+
+        current_center_x = (
+            self.camera_view.zoom_rect[0][0] + self.camera_view.zoom_rect[0][1]
+        ) / 2
+        current_center_y = (
+            self.camera_view.zoom_rect[1][0] + self.camera_view.zoom_rect[1][1]
+        ) / 2
+
+        self.camera_view.move_to_x = int(random.random())
+        self.camera_view.move_to_y = int(random.random())
+
+        # This is the formula to check
+        offset_x = (
+            (self.camera_view.move_to_x - current_center_x)
+            / self.camera_view.zoom_scale
+            * self.camera_view.canvas_width_scale
+            * pixel_size
+        )
+        offset_y = (
+            (self.camera_view.move_to_y - current_center_y)
+            / self.camera_view.zoom_scale
+            * self.camera_view.canvas_width_scale
+            * pixel_size
+        )
+
+        # Set the mode to check if statements
+        self.camera_view.mode = mode
+
+        # Act
+        self.camera_view.move_stage()
+
+        # Check
+        assert self.camera_view.parent_controller.pop() == "get_stage_position"
+        res = self.camera_view.parent_controller.pop()
+        if mode == "stop":
+            assert res == "move_stage_and_acquire_image"
+        else:
+            assert res == "move_stage_and_update_info"
+        # Checking that move stage properly changed pos
+        new_pos = self.camera_view.parent_controller.pop()
+        self.camera_view.parent_controller.stage_pos["x"] -= offset_x
+        self.camera_view.parent_controller.stage_pos["y"] += offset_y
+        assert new_pos == self.camera_view.parent_controller.stage_pos
+
+    def test_reset_display(self, monkeypatch):
+
+        # Mock process image function
+        process_image_called = False
+
+        def mock_process_image():
+            nonlocal process_image_called
+            process_image_called = True
+
+        monkeypatch.setattr(self.camera_view, "process_image", mock_process_image)
+
+        # Reset and check
+        self.camera_view.reset_display()
+        assert np.array_equal(
+            self.camera_view.zoom_rect,
+            np.array(
+                [
+                    [0, self.camera_view.view.canvas_width],
+                    [0, self.camera_view.view.canvas_height],
+                ]
+            ),
+        )
+        assert np.array_equal(self.camera_view.zoom_offset, np.array([[0], [0]]))
+        assert self.camera_view.zoom_value == 1
+        assert self.camera_view.zoom_scale == 1
+        assert self.camera_view.zoom_width == self.camera_view.view.canvas_width
+        assert self.camera_view.zoom_height == self.camera_view.view.canvas_height
+        assert process_image_called is True
+
+    def test_process_image(self):
+        self.camera_view.image = np.random.randint(0, 256, (600, 800))
+        self.camera_view.digital_zoom = MagicMock()
+        # self.camera_view.detect_saturation = MagicMock()
+        self.camera_view.down_sample_image = MagicMock()
+        self.camera_view.scale_image_intensity = MagicMock()
+        self.camera_view.add_crosshair = MagicMock()
+        self.camera_view.apply_lut = MagicMock()
+        self.camera_view.populate_image = MagicMock()
+
+        self.camera_view.process_image()
+
+        self.camera_view.digital_zoom.assert_called()
+        # self.camera_view.detect_saturation.assert_called()
+        self.camera_view.down_sample_image.assert_called()
+        self.camera_view.scale_image_intensity.assert_called()
+        self.camera_view.add_crosshair.assert_called()
+        self.camera_view.apply_lut.assert_called()
+        self.camera_view.populate_image.assert_called()
+
+    @pytest.mark.parametrize("num,value", [(4, 0.95), (5, 1.05)])
+    def test_mouse_wheel(self, num, value):
+
+        # Test zoom in and out
+        event = MagicMock()
+        event.num = num
+        event.x = int(random.random())
+        event.y = int(random.random())
+        event.delta = 120
+        self.camera_view.zoom_value = 1
+        self.camera_view.zoom_scale = 1
+        self.camera_view.zoom_width = self.camera_view.view.canvas_width
+        self.camera_view.zoom_height = self.camera_view.view.canvas_height
+        self.camera_view.reset_display = MagicMock()
+        self.camera_view.process_image = MagicMock()
+        self.camera_view.mouse_wheel(event)
+
+        assert self.camera_view.zoom_value == value
+        assert self.camera_view.zoom_scale == value
+        assert self.camera_view.zoom_width == self.camera_view.view.canvas_width / value
+        assert (
+            self.camera_view.zoom_height == self.camera_view.view.canvas_height / value
+        )
+
+        if (
+            self.camera_view.zoom_width > self.camera_view.view.canvas_width
+            or self.camera_view.zoom_height > self.camera_view.view.canvas_height
+        ):
+            assert self.camera_view.reset_display.called is True
+            self.camera_view.process_image.assert_called()
+        else:
+            assert self.camera_view.reset_display.called is False
+            not self.camera_view.process_image.assert_called()
+
+    @pytest.mark.skip("AssertionError: Expected 'mock' to have been called.")
+    def test_digital_zoom(self):
+
+        # Setup
+        a, b, c, d, e, f = [random.randint(1, 100) for _ in range(6)]
+        g, h = [random.randint(100, 400) for _ in range(2)]
+        i, j = [random.randint(500, 1000) for _ in range(2)]
+        val, scale, widthsc, heightsc = [random.randint(2, 4) for _ in range(4)]
+        self.camera_view.zoom_rect = np.array([[a, b], [c, d]])
+        self.camera_view.zoom_offset = np.array([[e], [f]])
+        self.camera_view.zoom_value = val
+        self.camera_view.zoom_scale = scale
+        self.camera_view.zoom_width = g  # 300
+        self.camera_view.zoom_height = h  # 400
+        self.camera_view.view.canvas_width = i  # 800
+        self.camera_view.view.canvas_height = j  # 600
+        self.camera_view.canvas_width_scale = widthsc
+        self.camera_view.canvas_height_scale = heightsc
+        self.camera_view.image = np.random.randint(0, 256, (600, 800))
+        self.camera_view.reset_display = MagicMock()
+
+        # Preprocess
+        new_zoom_rec = self.camera_view.zoom_rect - self.camera_view.zoom_offset
+        new_zoom_rec *= self.camera_view.zoom_value
+        new_zoom_rec += self.camera_view.zoom_offset
+
+        x_start_index = int(-new_zoom_rec[0][0] / self.camera_view.zoom_scale)
+        x_end_index = int(x_start_index + self.camera_view.zoom_width)
+        y_start_index = int(-new_zoom_rec[1][0] / self.camera_view.zoom_scale)
+        y_end_index = int(y_start_index + self.camera_view.zoom_height)
+
+        crosshair_x = (new_zoom_rec[0][0] + new_zoom_rec[0][1]) / 2
+        crosshair_y = (new_zoom_rec[1][0] + new_zoom_rec[1][1]) / 2
+        if crosshair_x < 0 or crosshair_x >= self.camera_view.view.canvas_width:
+            crosshair_x = -1
+        if crosshair_y < 0 or crosshair_y >= self.camera_view.view.canvas_height:
+            crosshair_y = -1
+
+        new_image = self.camera_view.image[
+            y_start_index
+            * self.camera_view.canvas_height_scale : y_end_index
+            * self.camera_view.canvas_height_scale,
+            x_start_index
+            * self.camera_view.canvas_width_scale : x_end_index
+            * self.camera_view.canvas_width_scale,
+        ]
+
+        # Call func
+        self.camera_view.digital_zoom()
+
+        # Check zoom rec
+        assert np.array_equal(self.camera_view.zoom_rect, new_zoom_rec)
+        # Check zoom offset
+        assert np.array_equal(self.camera_view.zoom_offset, np.array([[0], [0]]))
+        # Check zoom_value
+        assert self.camera_view.zoom_value == 1
+        # Check zoom_image
+        assert np.array_equal(self.camera_view.zoom_image, new_image)
+        # Check crosshairs
+        assert self.camera_view.crosshair_x == int(crosshair_x)
+        assert self.camera_view.crosshair_y == int(crosshair_y)
+        # Check reset display
+        self.camera_view.reset_display.assert_called()
+
+    @pytest.mark.parametrize("onoff", [True, False])
+    def test_left_click(self, onoff):
+        self.camera_view.add_crosshair = MagicMock()
+        self.camera_view.digital_zoom = MagicMock()
+        # self.camera_view.detect_saturation = MagicMock()
+        self.camera_view.down_sample_image = MagicMock()
+        self.camera_view.transpose_image = MagicMock()
+        self.camera_view.scale_image_intensity = MagicMock()
+        self.camera_view.apply_lut = MagicMock()
+        self.camera_view.populate_image = MagicMock()
+        event = MagicMock()
+
+        self.camera_view.image = np.random.randint(0, 256, (600, 800))
+        self.camera_view.apply_cross_hair = onoff
+
+        self.camera_view.left_click(event)
+
+        self.camera_view.add_crosshair.assert_called()
+        self.camera_view.populate_image.assert_called()
+        assert self.camera_view.apply_cross_hair == (not onoff)
+
+    @pytest.mark.parametrize("frames", [0, 1, 2, 10])
+    def test_update_max_count(self, frames):
+        self.camera_view.image_metrics["Frames"].set(frames)
+
+        idx = 0
+        temp = [0] * 40
+
+        while idx < 40:
+            self.camera_view._last_frame_display_max = np.random.randint(0, 500)
+            temp[idx] = self.camera_view._last_frame_display_max
+            idx += 1
+            # Act
+            self.camera_view.update_max_counts()
+
+            assert self.camera_view._max_intensity_history_idx == idx % 32
+            assert (
+                self.camera_view.max_intensity_history[
+                    self.camera_view._max_intensity_history_idx - 1
+                ]
+                == self.camera_view._last_frame_display_max
+            )
+
+            # Assert
+            if frames == 0:
+                assert self.camera_view.image_metrics["Frames"].get() == 1
+                frame_num = 1
+            else:
+                frame_num = frames
+
+            if frame_num <= idx:
+                rolling_average = sum(temp[idx - frame_num : idx]) / frame_num
+            else:
+                rolling_average = sum(temp[:idx]) / frame_num
+
+            assert self.camera_view.image_metrics["Image"].get() == round(
+                rolling_average, 0
+            )
+
+    def test_down_sample_image(self, monkeypatch):
+        import cv2
+
+        # create a test image
+        test_image = np.random.rand(100, 100)
+        self.zoom_image = test_image
+
+        # set the widget size
+        widget = type("MyWidget", (object,), {"widget": self.camera_view.view})
+        event = type(
+            "MyEvent",
+            (object,),
+            {
+                "widget": widget,
+                "width": np.random.randint(5, 1000),
+                "height": np.random.randint(5, 1000),
+            },
+        )
+        self.camera_view.resize(event)
+
+        # monkeypatch cv2.resize
+        def mocked_resize(src, dsize, interpolation=1):
+            return np.ones((dsize[0], dsize[1]))
+
+        monkeypatch.setattr(cv2, "resize", mocked_resize)
+
+        # call the function
+        down_sampled_image = self.camera_view.down_sample_image(test_image)
+
+        # assert that the image has been resized correctly
+        assert np.shape(down_sampled_image) == (
+            self.camera_view.view.canvas_width,
+            self.camera_view.view.canvas_height,
+        )
+
+        # assert that the image has not been modified
+        assert not np.array_equal(down_sampled_image, test_image)
+
+    @pytest.mark.parametrize("auto", [True, False])
+    def test_scale_image_intensity(self, auto):
+        # Create a test image
+        test_image = np.random.rand(100, 100)
+
+        # Set autoscale to True
+        self.camera_view.autoscale = auto
+
+        if auto is False:
+            self.camera_view.max_counts = 1.5
+            self.camera_view.min_counts = 0.5
+
+        # Call the function
+        scaled_image = self.camera_view.scale_image_intensity(test_image)
+
+        # Assert that max_counts and min_counts have been set correctly
+        if auto is True:
+            assert self.camera_view._last_frame_display_max == np.max(test_image)
+
+        # Assert that the image has been scaled correctly
+        assert np.min(scaled_image) >= 0
+        assert np.max(scaled_image) <= 255
+
+    def test_populate_image(self, monkeypatch):
+        from PIL import Image, ImageTk
+        import cv2
+
+        # Create test image
+        self.camera_view.cross_hair_image = np.random.rand(100, 100)
+        self.camera_view.ilastik_seg_mask = np.random.rand(100, 100)
+
+        # Set display_mask_flag to True
+        self.camera_view.display_mask_flag = True
+
+        # Monkeypatch the Image.fromarray() method of PIL
+        def mocked_fromarray(arr):
+            return arr
+
+        monkeypatch.setattr(Image, "fromarray", mocked_fromarray)
+
+        # Monkeypatch the cv2.resize() function
+        def mocked_resize(arr, size):
+            return arr
+
+        monkeypatch.setattr(cv2, "resize", mocked_resize)
+
+        # Monkeypatch the Image.blend() method of PIL
+        def mocked_blend(img1, img2, alpha):
+            return img1 * alpha + img2 * (1 - alpha)
+
+        monkeypatch.setattr(Image, "blend", mocked_blend)
+
+        def mocked_PhotoImage(img):
+            mock_photo = MagicMock()
+            # Set up width and height methods to return appropriate dimensions
+            mock_photo.width.return_value = 100
+            mock_photo.height.return_value = 100
+            return mock_photo
+
+        monkeypatch.setattr(ImageTk, "PhotoImage", mocked_PhotoImage)
+
+        self.camera_view.canvas.create_image = MagicMock()
+        self.camera_view.image_cache_flag = True
+
+        # Call the function
+        self.camera_view.populate_image(self.camera_view.cross_hair_image)
+
+        # Assert that the tk_image has been created correctly
+        assert self.camera_view._img_buf is not None
+
+        # Set display_mask_flag to True
+        self.camera_view.display_mask_flag = False
+
+        # Call the function
+        self.camera_view.populate_image(self.camera_view.cross_hair_image)
+
+    def test_initialize_non_live_display(self):
+        # Create test buffer and microscope_state
+        camera_parameters = {
+            "img_x_pixels": np.random.randint(1, 200),
+            "img_y_pixels": np.random.randint(1, 200),
+        }
+
+        # Call the function
+        self.camera_view.initialize_non_live_display(
+            self.microscope_state, camera_parameters
+        )
+
+        # Assert that the variables have been set correctly
+        assert self.camera_view.image_count == 0
+        assert self.camera_view.slice_index == 0
+        assert self.camera_view.number_of_channels == len(
+            self.microscope_state["channels"]
+        )
+        assert (
+            self.camera_view.number_of_slices == self.microscope_state["number_z_steps"]
+        )
+        assert (
+            self.camera_view.total_images_per_volume
+            == self.camera_view.number_of_channels * self.camera_view.number_of_slices
+        )
+        assert self.camera_view.original_image_width == int(
+            camera_parameters["img_x_pixels"]
+        )
+        assert self.camera_view.original_image_height == int(
+            camera_parameters["img_y_pixels"]
+        )
+        assert self.camera_view.canvas_width_scale == float(
+            self.camera_view.original_image_width / self.camera_view.canvas_width
+        )
+        assert self.camera_view.canvas_height_scale == float(
+            self.camera_view.original_image_height / self.camera_view.canvas_height
+        )
+
+    def test_identify_channel_index_and_slice(self):
+        # Not currently in use
+        pass
+
+    def test_retrieve_image_slice_from_volume(self):
+        # Not currently in use
+        pass
+
+    @pytest.mark.parametrize("transpose", [True, False])
+    def test_display_image(self, transpose):
+        """Test the display of an image on the camera view
+
+        TODO: The recent refactor makes this test non-functional. It needs to be updated
+        The newer code does not use the camera_view.image attribute after the
+        transpose operation, so any transpose/image flipping is not reflected in the
+        final image. The test should be updated to reflect this.,
+        """
+
+        self.camera_view.initialize_non_live_display(
+            self.microscope_state, {"img_x_pixels": 50, "img_y_pixels": 100}
+        )
+        self.camera_view.digital_zoom = MagicMock()
+        # self.camera_view.detect_saturation = MagicMock()
+        self.camera_view.down_sample_image = MagicMock()
+        self.camera_view.scale_image_intensity = MagicMock()
+        self.camera_view.apply_lut = MagicMock()
+        self.camera_view.populate_image = MagicMock()
+        images = np.random.rand(10, 100, 50)
+
+        self.camera_view.transpose = transpose
+        count = 0
+        self.camera_view.image_count = count
+        self.camera_view.image_metrics = {"Channel": MagicMock()}
+        self.camera_view.update_max_counts = MagicMock()
+        self.camera_view.flip_flags = {"x": False, "y": False}
+
+        image_id = np.random.randint(0, 10)
+        self.camera_view.try_to_display_image(images[image_id])
+
+        assert (
+            self.camera_view.spooled_images.size_y,
+            self.camera_view.spooled_images.size_x,
+        ) == np.shape(images[image_id])
+        assert self.camera_view.image_count == count + 1
+
+        self.camera_view.flip_flags = {"x": True, "y": False}
+        image_id = np.random.randint(0, 10)
+        self.camera_view.try_to_display_image(images[image_id])
+        # assert np.shape(self.camera_view.image) == np.shape(images[image_id])
+        # if not transpose:
+        #     assert (self.camera_view.image == images[image_id][:, ::-1]).all()
+        # else:
+        #     assert (self.camera_view.image == images[image_id][:, ::-1].T).all()
+        assert self.camera_view.image_count == count + 2
+
+        self.camera_view.flip_flags = {"x": False, "y": True}
+        image_id = np.random.randint(0, 10)
+        self.camera_view.try_to_display_image(images[image_id])
+        # assert np.shape(self.camera_view.image) == np.shape(images[image_id])
+        # if not transpose:
+        #     assert (self.camera_view.image == images[image_id][::-1, :]).all()
+        # else:
+        #     assert (self.camera_view.image == images[image_id][::-1, :].T).all()
+        assert self.camera_view.image_count == count + 3
+
+        self.camera_view.flip_flags = {"x": True, "y": True}
+        image_id = np.random.randint(0, 10)
+        self.camera_view.try_to_display_image(images[image_id])
+        # assert np.shape(self.camera_view.image) == np.shape(images[image_id])
+        # if not transpose:
+        #     assert (self.camera_view.image == images[image_id][::-1, ::-1]).all()
+        # else:
+        #     assert (self.camera_view.image == images[image_id][::-1, ::-1].T).all()
+        assert self.camera_view.image_count == count + 4
+
+    def test_add_crosshair(self):
+
+        # Arrange
+        x = self.camera_view.canvas_width
+        y = self.camera_view.canvas_height
+        image = np.random.rand(x, y)
+        self.camera_view.apply_cross_hair = True
+
+        # Act
+        image2 = self.camera_view.add_crosshair(image)
+
+        # Assert
+        assert np.all(image2[:, self.camera_view.zoom_rect[0][1] // 2] == 255)
+        assert np.all(image2[self.camera_view.zoom_rect[1][1] // 2, :] == 255)
+
+    def test_apply_LUT(self):
+        # Someone else with better numpy understanding will need to do this TODO
+
+        pass
+
+    def test_update_LUT(self):
+        # Same as apply LUT TODO
+        pass
+
+    def test_toggle_min_max_button(self):
+
+        # Setup for true path
+        self.camera_view.image_palette["Autoscale"].set(True)
+
+        # Act by calling function
+        self.camera_view.toggle_min_max_buttons()
+
+        # Assert things are correct
+        assert str(self.camera_view.image_palette["Min"].widget["state"]) == "disabled"
+        assert str(self.camera_view.image_palette["Max"].widget["state"]) == "disabled"
+
+        # Setup for false path
+        self.camera_view.image_palette["Autoscale"].set(False)
+
+        # Mock function call to isolate
+        self.camera_view.update_min_max_counts = MagicMock()
+
+        # Act by calling function
+        self.camera_view.toggle_min_max_buttons()
+
+        # Assert things are correct and called
+        assert str(self.camera_view.image_palette["Min"].widget["state"]) == "normal"
+        assert str(self.camera_view.image_palette["Max"].widget["state"]) == "normal"
+        self.camera_view.update_min_max_counts.assert_called()
+
+    def test_transpose_image(self):
+        # Create test data
+        self.camera_view.image_palette["Flip XY"].set(True)
+        self.camera_view.transpose = None
+
+        # Call the function
+        self.camera_view.update_transpose_state()
+
+        # Assert the output
+        assert self.camera_view.transpose is True
+
+        # Create test data
+        self.camera_view.image_palette["Flip XY"].set(False)
+        self.camera_view.transpose = None
+
+        # Call the function
+        self.camera_view.update_transpose_state()
+
+        # Assert the output
+        assert self.camera_view.transpose is False
+
+    def test_update_min_max_counts(self):
+        # Create test data
+        min = np.random.randint(0, 10)
+        max = np.random.randint(0, 10)
+        self.camera_view.image_palette["Min"].set(min)
+        self.camera_view.image_palette["Max"].set(max)
+
+        self.camera_view.min_counts = None
+        self.camera_view.max_counts = None
+
+        # Call the function
+        self.camera_view.update_min_max_counts()
+
+        # Assert the output
+        assert self.camera_view.min_counts == min
+        assert self.camera_view.max_counts == max
+
+    def test_set_mask_color_table(self):
+        # This is beyond me currently TODO
+        pass
+
+    def test_display_mask(self, monkeypatch):
+        import cv2
+
+        # Create test data
+        self.camera_view.ilastik_seg_mask = None
+        self.camera_view.ilastik_mask_ready_lock.acquire()
+        mask = np.zeros((5, 5), dtype=np.uint8)
+        self.camera_view.mask_color_table = np.zeros((256, 1, 3), dtype=np.uint8)
+
+        # Define the monkeypatch
+        def mock_applyColorMap(mask, mask_color_table):
+            return mask
+
+        # Apply the monkeypatch
+        monkeypatch.setattr(cv2, "applyColorMap", mock_applyColorMap)
+
+        # Call the function
+        self.camera_view.display_mask(mask)
+
+        # Assert the output
+        assert (self.camera_view.ilastik_seg_mask == mask).all()
+        assert not self.camera_view.ilastik_mask_ready_lock.locked()
+
+    def test_update_canvas_size(self):
+        self.camera_view.view.canvas["width"] = random.randint(1, 2000)
+        self.camera_view.view.canvas["height"] = random.randint(1, 2000)
+
+        self.camera_view.update_canvas_size()
+
+        assert self.camera_view.canvas_width > 0
+        assert self.camera_view.canvas_height > 0

test/controller/sub_controllers/test_channels_settings.py

@@ -0,0 +1,168 @@
+# Copyright (c) 2021-2025  The University of Texas Southwestern Medical Center.
+# All rights reserved.
+
+# Redistribution and use in source and binary forms, with or without
+# modification, are permitted for academic and research use only (subject to the
+# limitations in the disclaimer below) provided that the following conditions are met:
+
+#      * Redistributions of source code must retain the above copyright notice,
+#      this list of conditions and the following disclaimer.
+
+#      * Redistributions in binary form must reproduce the above copyright
+#      notice, this list of conditions and the following disclaimer in the
+#      documentation and/or other materials provided with the distribution.
+
+#      * Neither the name of the copyright holders nor the names of its
+#      contributors may be used to endorse or promote products derived from this
+#      software without specific prior written permission.
+
+# NO EXPRESS OR IMPLIED LICENSES TO ANY PARTY'S PATENT RIGHTS ARE GRANTED BY
+# THIS LICENSE. THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND
+# CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT
+# LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
+# PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT HOLDER OR
+# CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL,
+# EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO,
+# PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR
+# BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER
+# IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE)
+# ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE
+# POSSIBILITY OF SUCH DAMAGE.
+#
+import pytest
+
+
+class TestChannelSettingController:
+    @pytest.fixture(autouse=True)
+    def setup_class(self, dummy_controller):
+        from navigate.controller.sub_controllers.channels_tab import (
+            ChannelsTabController,
+        )
+        from navigate.controller.sub_controllers.channels_settings import (
+            ChannelSettingController,
+        )
+
+        self.ctc = ChannelsTabController(
+            dummy_controller.view.settings.channels_tab, dummy_controller
+        )
+        self.ctc.commands = []
+        self.ctc.execute = lambda command: self.ctc.commands.append(command)
+
+        self.channel_setting = ChannelSettingController(
+            self.ctc.view.channel_widgets_frame,
+            self.ctc,
+            dummy_controller.configuration_controller,
+        )
+        self.channel_setting.populate_experiment_values(
+            dummy_controller.configuration["experiment"]["MicroscopeState"]["channels"]
+        )
+
+    @pytest.mark.parametrize(
+        "mode,state,state_readonly",
+        [("stop", "normal", "readonly"), ("live", "disabled", "readonly")],
+    )
+    def test_set_mode(self, mode, state, state_readonly):
+
+        self.channel_setting.set_mode(mode)
+
+        for i in range(5):
+            assert str(self.channel_setting.view.channel_checks[i]["state"]) == state
+            # interval widget is disabled now
+            assert (
+                str(self.channel_setting.view.interval_spins[i]["state"]) == "disabled"
+            )
+            if mode == "stop":
+                assert (
+                    str(self.channel_setting.view.laser_pulldowns[i]["state"])
+                    == state_readonly
+                )
+            else:
+                assert (
+                    str(self.channel_setting.view.laser_pulldowns[i]["state"])
+                    == "disabled"
+                )
+            if self.channel_setting.mode != "live" or (
+                self.channel_setting.mode == "live"
+                and not self.channel_setting.view.channel_variables[i].get()
+            ):
+                assert (
+                    str(self.channel_setting.view.exptime_pulldowns[i]["state"])
+                    == state
+                )
+            if not self.channel_setting.view.channel_variables[i].get():
+                assert (
+                    str(self.channel_setting.view.laserpower_pulldowns[i]["state"])
+                    == state
+                )
+                assert (
+                    str(self.channel_setting.view.filterwheel_pulldowns[i]["state"])
+                    == state_readonly
+                )
+                assert str(self.channel_setting.view.defocus_spins[i]["state"]) == state
+
+    def test_channel_callback(self):
+        import random
+
+        self.channel_setting.in_initialization = False
+        channel_dict = (
+            self.channel_setting.parent_controller.parent_controller.configuration[
+                "experiment"
+            ]["MicroscopeState"]["channels"]
+        )
+        # shuffle the channels
+        new_channel_dict = {
+            k: v
+            for k, v in zip(
+                channel_dict.keys(),
+                random.choices(channel_dict.values(), k=len(channel_dict.keys())),
+            )
+        }
+
+        self.channel_setting.populate_experiment_values(channel_dict)
+        for channel_id in range(self.channel_setting.num):
+            vals = self.channel_setting.get_vals_by_channel(channel_id)
+            channel_key = f"channel_{str(channel_id + 1)}"
+            try:
+                setting_dict = channel_dict[channel_key]
+                new_setting_dict = new_channel_dict[channel_key]
+            except KeyError:
+                continue
+
+            # Test channel callback through trace writes
+            for k in setting_dict.keys():
+                if k == "laser_index" or k.startswith("filter_position"):
+                    continue
+                if k == "defocus":
+                    new_val = float(random.randint(1, 10))
+                else:
+                    new_val = new_setting_dict[k]
+
+                vals[k].set(new_val)
+
+                assert str(vals[k].get()) == str(new_val)
+                if k != "defocus":
+                    assert setting_dict[k] == new_setting_dict[k]
+
+                if k == "laser":
+                    assert (
+                        setting_dict["laser_index"] == new_setting_dict["laser_index"]
+                    )
+                elif k == "filter":
+                    assert (
+                        setting_dict["filter_position"]
+                        == new_setting_dict["filter_position"]
+                    )
+                elif k == "camera_exposure_time" or k == "is_selected":
+                    assert (
+                        self.channel_setting.parent_controller.commands.pop()
+                        == "recalculate_timepoint"
+                    )
+                    self.channel_setting.parent_controller.commands = []  # reset
+
+    def test_get_vals_by_channel(self):
+        # Not needed to test IMO
+        pass
+
+    def test_get_index(self):
+        # Not needed to test IMO
+        pass

test/controller/sub_controllers/test_channels_tab.py

@@ -0,0 +1,385 @@
+# Copyright (c) 2021-2025  The University of Texas Southwestern Medical Center.
+# All rights reserved.
+
+# Redistribution and use in source and binary forms, with or without
+# modification, are permitted for academic and research use only (subject to the
+# limitations in the disclaimer below) provided that the following conditions are met:
+
+#      * Redistributions of source code must retain the above copyright notice,
+#      this list of conditions and the following disclaimer.
+
+#      * Redistributions in binary form must reproduce the above copyright
+#      notice, this list of conditions and the following disclaimer in the
+#      documentation and/or other materials provided with the distribution.
+
+#      * Neither the name of the copyright holders nor the names of its
+#      contributors may be used to endorse or promote products derived from this
+#      software without specific prior written permission.
+
+# NO EXPRESS OR IMPLIED LICENSES TO ANY PARTY'S PATENT RIGHTS ARE GRANTED BY
+# THIS LICENSE. THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND
+# CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT
+# LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
+# PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT HOLDER OR
+# CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL,
+# EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO,
+# PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR
+# BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER
+# IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE)
+# ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE
+# POSSIBILITY OF SUCH DAMAGE.
+#
+
+import random
+import copy
+
+import pytest
+import numpy as np
+from unittest.mock import patch
+
+
+@pytest.fixture
+def channels_tab_controller(dummy_controller):
+    from navigate.controller.sub_controllers.channels_tab import (
+        ChannelsTabController,
+    )
+
+    return ChannelsTabController(
+        dummy_controller.view.settings.channels_tab, dummy_controller
+    )
+
+
+def test_update_z_steps(channels_tab_controller):
+    # Calculate params
+    z_start, f_start = random.randint(1, 1000), random.randint(1, 1000)
+    z_end, f_end = random.randint(1, 1000), random.randint(1, 1000)
+    if z_end < z_start:
+        # Sort so we are always going low to high
+        tmp = z_start
+        tmp_f = f_start
+        z_start = z_end
+        f_start = f_end
+        z_end = tmp
+        f_end = tmp_f
+    step_size = max(1, min(random.randint(1, 10), (z_end - z_start) // 2))
+
+    # Set params
+    channels_tab_controller.microscope_state_dict = (
+        channels_tab_controller.parent_controller.configuration["experiment"][
+            "MicroscopeState"
+        ]
+    )
+    channels_tab_controller.in_initialization = False
+    channels_tab_controller.stack_acq_vals["start_position"].set(z_start)
+    channels_tab_controller.stack_acq_vals["start_focus"].set(f_start)
+    channels_tab_controller.stack_acq_vals["end_position"].set(z_end)
+    channels_tab_controller.stack_acq_vals["end_focus"].set(f_end)
+    channels_tab_controller.stack_acq_vals["step_size"].set(step_size)
+
+    # Run
+    channels_tab_controller.update_z_steps()
+
+    # Verify
+    number_z_steps = int(np.ceil(np.abs((z_start - z_end) / step_size)))
+    assert (
+        int(channels_tab_controller.stack_acq_vals["number_z_steps"].get())
+        == number_z_steps
+    )
+
+    # test flip_z is True
+    microscope_name = (
+        channels_tab_controller.parent_controller.configuration_controller.microscope_name
+    )
+    stage_config = channels_tab_controller.parent_controller.configuration[
+        "configuration"
+    ]["microscopes"][microscope_name]["stage"]
+    stage_config["flip_z"] = True
+    channels_tab_controller.z_origin = (z_start + z_end) / 2
+    channels_tab_controller.stack_acq_vals["start_position"].set(z_end)
+    channels_tab_controller.stack_acq_vals["start_focus"].set(f_end)
+    channels_tab_controller.stack_acq_vals["end_position"].set(z_start)
+    channels_tab_controller.stack_acq_vals["end_focus"].set(f_start)
+    channels_tab_controller.update_z_steps()
+    assert channels_tab_controller.stack_acq_vals["step_size"].get() == step_size
+    assert channels_tab_controller.microscope_state_dict["step_size"] == -1 * step_size
+    assert (
+        channels_tab_controller.stack_acq_vals["number_z_steps"].get() == number_z_steps
+    )
+    stage_config["flip_z"] = False
+
+
+def test_update_start_position(channels_tab_controller):
+    z, f = random.randint(0, 1000), random.randint(0, 1000)
+    channels_tab_controller.parent_controller.configuration["experiment"][
+        "StageParameters"
+    ]["z"] = z
+    channels_tab_controller.parent_controller.configuration["experiment"][
+        "StageParameters"
+    ]["f"] = f
+
+    channels_tab_controller.update_start_position()
+
+    assert channels_tab_controller.z_origin == z
+    assert channels_tab_controller.focus_origin == f
+    assert int(channels_tab_controller.stack_acq_vals["start_position"].get()) == 0
+    assert int(channels_tab_controller.stack_acq_vals["start_focus"].get()) == 0
+
+    # test flip_z is True
+    microscope_name = (
+        channels_tab_controller.parent_controller.configuration_controller.microscope_name
+    )
+    stage_config = channels_tab_controller.parent_controller.configuration[
+        "configuration"
+    ]["microscopes"][microscope_name]["stage"]
+    stage_config["flip_z"] = True
+    channels_tab_controller.update_start_position()
+
+    assert channels_tab_controller.z_origin == z
+    assert channels_tab_controller.focus_origin == f
+    assert int(channels_tab_controller.stack_acq_vals["end_position"].get()) == 0
+    assert int(channels_tab_controller.stack_acq_vals["end_focus"].get()) == 0
+    stage_config["flip_z"] = False
+
+
+def test_update_end_position(channels_tab_controller):
+    configuration = channels_tab_controller.parent_controller.configuration
+
+    # Initialize
+    z, f = random.randint(0, 1000), random.randint(0, 1000)
+    z_shift, f_shift = random.randint(1, 500), random.randint(1, 500)
+    configuration["experiment"]["StageParameters"]["z"] = z + z_shift
+    configuration["experiment"]["StageParameters"]["f"] = f + f_shift
+
+    print(f"z: {z} z-shift: {z_shift} f: {f} f-shift: {f_shift}")
+    print(f'z-dict: {configuration["experiment"]["StageParameters"]["z"]}')
+    print(f'f-dict: {configuration["experiment"]["StageParameters"]["f"]}')
+
+    # Step backwards and record results
+    channels_tab_controller.z_origin = z - z_shift
+    channels_tab_controller.focus_origin = f - f_shift
+    channels_tab_controller.update_end_position()
+    z_origin_minus = copy.deepcopy(channels_tab_controller.z_origin)
+    f_origin_minus = copy.deepcopy(channels_tab_controller.focus_origin)
+    start_position_minus = copy.deepcopy(
+        channels_tab_controller.stack_acq_vals["start_position"].get()
+    )
+    end_position_minus = copy.deepcopy(
+        channels_tab_controller.stack_acq_vals["end_position"].get()
+    )
+    start_focus_minus = copy.deepcopy(
+        channels_tab_controller.stack_acq_vals["start_focus"].get()
+    )
+    end_focus_minus = copy.deepcopy(
+        channels_tab_controller.stack_acq_vals["end_focus"].get()
+    )
+
+    print("back")
+    print(f"z: {z} z-shift: {z_shift} f: {f} f-shift: {f_shift}")
+    print(f'z-dict: {configuration["experiment"]["StageParameters"]["z"]}')
+    print(f'f-dict: {configuration["experiment"]["StageParameters"]["f"]}')
+
+    # Step forward
+    configuration["experiment"]["StageParameters"]["z"] = z - z_shift
+    configuration["experiment"]["StageParameters"]["f"] = f - f_shift
+    channels_tab_controller.z_origin = z + z_shift
+    channels_tab_controller.focus_origin = f + f_shift
+    channels_tab_controller.update_end_position()
+
+    print("forward")
+    print(f"z: {z} z-shift: {z_shift} f: {f} f-shift: {f_shift}")
+    print(f'z-dict: {configuration["experiment"]["StageParameters"]["z"]}')
+    print(f'f-dict: {configuration["experiment"]["StageParameters"]["f"]}')
+
+    # Ensure we achieve the same origin
+    assert channels_tab_controller.z_origin == z_origin_minus
+    assert channels_tab_controller.focus_origin == f_origin_minus
+    assert (
+        channels_tab_controller.stack_acq_vals["start_position"].get()
+        == start_position_minus
+    )
+    assert (
+        channels_tab_controller.stack_acq_vals["end_position"].get()
+        == end_position_minus
+    )
+    assert (
+        channels_tab_controller.stack_acq_vals["start_focus"].get() == start_focus_minus
+    )
+    assert channels_tab_controller.stack_acq_vals["end_focus"].get() == end_focus_minus
+
+    # test flip_z is True
+    microscope_name = (
+        channels_tab_controller.parent_controller.configuration_controller.microscope_name
+    )
+    stage_config = channels_tab_controller.parent_controller.configuration[
+        "configuration"
+    ]["microscopes"][microscope_name]["stage"]
+    stage_config["flip_z"] = True
+    # forward
+    channels_tab_controller.z_origin = z
+    channels_tab_controller.focus_origin = f
+    configuration["experiment"]["StageParameters"]["z"] = z - 2 * z_shift
+    configuration["experiment"]["StageParameters"]["f"] = f - 2 * f_shift
+    channels_tab_controller.update_end_position()
+    assert channels_tab_controller.z_origin == z - z_shift
+    assert channels_tab_controller.focus_origin == f - f_shift
+    assert channels_tab_controller.stack_acq_vals["start_position"].get() == z_shift
+    assert channels_tab_controller.stack_acq_vals["end_position"].get() == -1 * z_shift
+    assert channels_tab_controller.stack_acq_vals["start_focus"].get() == f_shift
+    assert channels_tab_controller.stack_acq_vals["end_focus"].get() == -1 * f_shift
+
+    # backward
+    channels_tab_controller.z_origin = z
+    channels_tab_controller.focus_origin = f
+    configuration["experiment"]["StageParameters"]["z"] = z + 2 * z_shift
+    configuration["experiment"]["StageParameters"]["f"] = f + 2 * f_shift
+    channels_tab_controller.update_end_position()
+    assert channels_tab_controller.z_origin == z + z_shift
+    assert channels_tab_controller.focus_origin == f + f_shift
+    assert channels_tab_controller.stack_acq_vals["start_position"].get() == z_shift
+    assert channels_tab_controller.stack_acq_vals["end_position"].get() == -1 * z_shift
+    assert channels_tab_controller.stack_acq_vals["start_focus"].get() == f_shift
+    assert channels_tab_controller.stack_acq_vals["end_focus"].get() == -1 * f_shift
+    stage_config["flip_z"] = False
+
+
+def test_update_start_update_end_position(channels_tab_controller):
+    configuration = channels_tab_controller.parent_controller.configuration
+    channels_tab_controller.microscope_state_dict = configuration["experiment"][
+        "MicroscopeState"
+    ]
+    channels_tab_controller.in_initialization = False
+
+    # Initialize
+    z, f = random.randint(0, 1000), random.randint(0, 1000)
+    z_shift, f_shift = random.randint(1, 500), random.randint(1, 500)
+    configuration["experiment"]["StageParameters"]["z"] = z - z_shift
+    configuration["experiment"]["StageParameters"]["f"] = f - f_shift
+    channels_tab_controller.update_start_position()
+
+    print(f"z: {z} z-shift: {z_shift} f: {f} f-shift: {f_shift}")
+    print(f'z-dict: {configuration["experiment"]["StageParameters"]["z"]}')
+    print(f'f-dict: {configuration["experiment"]["StageParameters"]["f"]}')
+
+    # Step forward and record results
+    configuration["experiment"]["StageParameters"]["z"] = z + z_shift
+    configuration["experiment"]["StageParameters"]["f"] = f + f_shift
+    channels_tab_controller.update_end_position()
+    z_origin_minus = copy.deepcopy(channels_tab_controller.z_origin)
+    f_origin_minus = copy.deepcopy(channels_tab_controller.focus_origin)
+    start_position_minus = copy.deepcopy(
+        channels_tab_controller.stack_acq_vals["start_position"].get()
+    )
+    end_position_minus = copy.deepcopy(
+        channels_tab_controller.stack_acq_vals["end_position"].get()
+    )
+    start_focus_minus = copy.deepcopy(
+        channels_tab_controller.stack_acq_vals["start_focus"].get()
+    )
+    end_focus_minus = copy.deepcopy(
+        channels_tab_controller.stack_acq_vals["end_focus"].get()
+    )
+
+    print("back")
+    print(f"z: {z} z-shift: {z_shift} f: {f} f-shift: {f_shift}")
+    print(f'z-dict: {configuration["experiment"]["StageParameters"]["z"]}')
+    print(f'f-dict: {configuration["experiment"]["StageParameters"]["f"]}')
+
+    channels_tab_controller.update_start_position()
+
+    # Step back
+    configuration["experiment"]["StageParameters"]["z"] = z - z_shift
+    configuration["experiment"]["StageParameters"]["f"] = f - f_shift
+    channels_tab_controller.update_end_position()
+
+    print("forward")
+    print(f"z: {z} z-shift: {z_shift} f: {f} f-shift: {f_shift}")
+    print(f'z-dict: {configuration["experiment"]["StageParameters"]["z"]}')
+    print(f'f-dict: {configuration["experiment"]["StageParameters"]["f"]}')
+
+    # Ensure we achieve the same origin
+    assert channels_tab_controller.z_origin == z_origin_minus
+    assert channels_tab_controller.focus_origin == f_origin_minus
+    assert (
+        channels_tab_controller.stack_acq_vals["start_position"].get()
+        == start_position_minus
+    )
+    assert (
+        channels_tab_controller.stack_acq_vals["end_position"].get()
+        == end_position_minus
+    )
+    assert (
+        channels_tab_controller.stack_acq_vals["start_focus"].get() == start_focus_minus
+    )
+    assert channels_tab_controller.stack_acq_vals["end_focus"].get() == end_focus_minus
+
+    # test flip_z is true
+    microscope_name = (
+        channels_tab_controller.parent_controller.configuration_controller.microscope_name
+    )
+    stage_config = channels_tab_controller.parent_controller.configuration[
+        "configuration"
+    ]["microscopes"][microscope_name]["stage"]
+    stage_config["flip_z"] = True
+    configuration = channels_tab_controller.parent_controller.configuration
+    z, f = random.randint(0, 1000), random.randint(0, 1000)
+    z_shift, f_shift = random.randint(1, 500), random.randint(1, 500)
+    configuration["experiment"]["StageParameters"]["z"] = z - z_shift
+    configuration["experiment"]["StageParameters"]["f"] = f - f_shift
+    channels_tab_controller.update_start_position()
+    configuration["experiment"]["StageParameters"]["z"] = z + z_shift
+    configuration["experiment"]["StageParameters"]["f"] = f + f_shift
+    channels_tab_controller.update_end_position()
+
+    assert channels_tab_controller.z_origin == z
+    assert channels_tab_controller.focus_origin == f
+    assert channels_tab_controller.stack_acq_vals["start_position"].get() == z_shift
+    assert channels_tab_controller.stack_acq_vals["end_position"].get() == -1 * z_shift
+    assert channels_tab_controller.stack_acq_vals["start_focus"].get() == f_shift
+    assert channels_tab_controller.stack_acq_vals["end_focus"].get() == -1 * f_shift
+
+    assert configuration["experiment"]["MicroscopeState"]["start_position"] == z_shift
+    assert (
+        configuration["experiment"]["MicroscopeState"]["end_position"] == -1 * z_shift
+    )
+    assert configuration["experiment"]["MicroscopeState"]["abs_z_start"] == z - z_shift
+    assert configuration["experiment"]["MicroscopeState"]["abs_z_end"] == z + z_shift
+    assert configuration["experiment"]["MicroscopeState"]["start_focus"] == f_shift
+    assert configuration["experiment"]["MicroscopeState"]["end_focus"] == -1 * f_shift
+
+    configuration["experiment"]["StageParameters"]["z"] = z + z_shift
+    configuration["experiment"]["StageParameters"]["f"] = f + f_shift
+    channels_tab_controller.update_start_position()
+    configuration["experiment"]["StageParameters"]["z"] = z - z_shift
+    configuration["experiment"]["StageParameters"]["f"] = f - f_shift
+    channels_tab_controller.update_end_position()
+
+    assert channels_tab_controller.z_origin == z
+    assert channels_tab_controller.focus_origin == f
+    assert channels_tab_controller.stack_acq_vals["start_position"].get() == z_shift
+    assert channels_tab_controller.stack_acq_vals["end_position"].get() == -1 * z_shift
+    assert channels_tab_controller.stack_acq_vals["start_focus"].get() == f_shift
+    assert channels_tab_controller.stack_acq_vals["end_focus"].get() == -1 * f_shift
+
+    assert configuration["experiment"]["MicroscopeState"]["start_position"] == z_shift
+    assert (
+        configuration["experiment"]["MicroscopeState"]["end_position"] == -1 * z_shift
+    )
+    assert configuration["experiment"]["MicroscopeState"]["abs_z_start"] == z - z_shift
+    assert configuration["experiment"]["MicroscopeState"]["abs_z_end"] == z + z_shift
+    assert configuration["experiment"]["MicroscopeState"]["start_focus"] == f_shift
+    assert configuration["experiment"]["MicroscopeState"]["end_focus"] == -1 * f_shift
+    stage_config["flip_z"] = False
+
+
+@pytest.mark.parametrize("is_multiposition", [True, False])
+def test_toggle_multiposition(channels_tab_controller, is_multiposition):
+    channels_tab_controller.populate_experiment_values()
+    channels_tab_controller.is_multiposition_val.set(is_multiposition)
+    with patch.object(channels_tab_controller, "update_timepoint_setting") as uts:
+        channels_tab_controller.toggle_multiposition()
+        assert channels_tab_controller.is_multiposition == is_multiposition
+        assert (
+            channels_tab_controller.microscope_state_dict["is_multiposition"]
+            == is_multiposition
+        )
+        uts.assert_called()

test/controller/sub_controllers/test_menus.py

@@ -0,0 +1,259 @@
+# Copyright (c) 2021-2025  The University of Texas Southwestern Medical Center.
+# All rights reserved.
+
+# Redistribution and use in source and binary forms, with or without
+# modification, are permitted for academic and research use only
+# (subject to the limitations in the disclaimer below)
+# provided that the following conditions are met:
+
+#      * Redistributions of source code must retain the above copyright notice,
+#      this list of conditions and the following disclaimer.
+
+#      * Redistributions in binary form must reproduce the above copyright
+#      notice, this list of conditions and the following disclaimer in the
+#      documentation and/or other materials provided with the distribution.
+
+#      * Neither the name of the copyright holders nor the names of its
+#      contributors may be used to endorse or promote products derived from this
+#      software without specific prior written permission.
+
+# NO EXPRESS OR IMPLIED LICENSES TO ANY PARTY'S PATENT RIGHTS ARE GRANTED BY
+# THIS LICENSE. THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND
+# CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT
+# LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
+# PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT HOLDER OR
+# CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL,
+# EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO,
+# PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR
+# BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER
+# IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE)
+# ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE
+# POSSIBILITY OF SUCH DAMAGE.
+
+# Standard Library Imports
+import unittest
+from unittest.mock import MagicMock, patch
+import tkinter as tk
+
+# Third Party Imports
+import pytest
+
+# Local Imports
+from navigate.controller.sub_controllers.menus import (
+    MenuController,
+    FakeEvent,
+)
+
+
+class TestFakeEvent(unittest.TestCase):
+    def test_fake_event_creation(self):
+        fake_event = FakeEvent(char="a", keysym="A")
+        self.assertEqual(fake_event.char, "a")
+        self.assertEqual(fake_event.keysym, "A")
+        self.assertEqual(fake_event.state, 0)
+
+
+class TestStageMovement(unittest.TestCase):
+    def setUp(self):
+        # Create a mock parent controller and view
+        self.root = tk.Tk()
+        self.parent_controller = MagicMock()
+        self.parent_controller.stage_controller = MagicMock()
+        self.view = MagicMock()
+        self.view.root = self.root
+
+        # Initialize the menu controller
+        self.mc = MenuController(self.view, self.parent_controller)
+
+        # Mock the histogram configuration entry.
+        self.parent_controller.configuration["gui"]["histogram"] = MagicMock()
+        self.parent_controller.configuration["gui"]["histogram"].get.return_value = True
+
+    def tearDown(self):
+        self.root.destroy()
+
+    def test_initialize_menus(self):
+        self.mc.initialize_menus()
+
+    def test_stage_movement_with_ttk_entry(self):
+        self.mc.parent_controller.view.focus_get.return_value = MagicMock(
+            widgetName="ttk::entry"
+        )
+        self.mc.stage_movement("a")
+        self.mc.parent_controller.stage_controller.stage_key_press.assert_not_called()
+
+    def test_stage_movement_with_ttk_combobox(self):
+        self.mc.parent_controller.view.focus_get.return_value = MagicMock(
+            widgetName="ttk::combobox"
+        )
+        self.mc.stage_movement("a")
+        self.mc.parent_controller.stage_controller.stage_key_press.assert_not_called()
+
+    def test_stage_movement_with_other_widget(self):
+        self.mc.parent_controller.view.focus_get.return_value = MagicMock(
+            widgetName="other_widget"
+        )
+        self.mc.stage_movement("a")
+        self.mc.parent_controller.stage_controller.stage_key_press.assert_called_with(
+            self.mc.fake_event
+        )
+
+    def test_stage_movement_with_key_error(self):
+        self.mc.parent_controller.view.focus_get.side_effect = KeyError
+        # Test that no exception is raised
+        try:
+            self.mc.stage_movement("a")
+        except KeyError:
+            self.fail("stage_movement() raised KeyError unexpectedly!")
+
+    def test_stage_movement_with_no_focus(self):
+        self.mc.parent_controller.view.focus_get.return_value = None
+        self.mc.stage_movement("a")
+        self.mc.parent_controller.stage_controller.stage_key_press.assert_called_with(
+            self.mc.fake_event
+        )
+
+
+class TestMenuController(unittest.TestCase):
+    @pytest.fixture(autouse=True)
+    def setup_class(self, dummy_controller):
+        c = dummy_controller
+        v = dummy_controller.view
+        self.menu_controller = MenuController(v, c)
+
+    def test_attributes(self):
+        methods = dir(MenuController)
+        desired_methods = [
+            "initialize_menus",
+            "populate_menu",
+            "new_experiment",
+            "load_experiment",
+            "save_experiment",
+            "load_images",
+            "popup_camera_map_setting",
+            "popup_ilastik_setting",
+            "popup_help",
+            "toggle_stage_limits",
+            "popup_autofocus_setting",
+            "popup_waveform_setting",
+            "popup_microscope_setting",
+            "toggle_save",
+            "acquire_data",
+            "not_implemented",
+            "stage_movement",
+            "switch_tabs",
+        ]
+
+        for method in desired_methods:
+            assert method in methods
+
+    def test_popup_camera_map_setting(self):
+        assert (
+            hasattr(
+                self.menu_controller.parent_controller, "camera_map_popup_controller"
+            )
+            is False
+        )
+        self.menu_controller.popup_camera_map_setting()
+        assert (
+            hasattr(
+                self.menu_controller.parent_controller, "camera_map_popup_controller"
+            )
+            is True
+        )
+
+    def test_autofocus_settings(self):
+        assert (
+            hasattr(self.menu_controller.parent_controller, "af_popup_controller")
+            is False
+        )
+        self.menu_controller.popup_autofocus_setting()
+        assert (
+            hasattr(self.menu_controller.parent_controller, "af_popup_controller")
+            is True
+        )
+
+    def test_popup_waveform_setting(self):
+        # TODO: Incomplete.
+        assert (
+            hasattr(self.menu_controller.parent_controller, "waveform_popup_controller")
+            is False
+        )
+
+    def test_popup_microscope_setting(self):
+        # TODO: Incomplete. DummyController has no attribute 'model'
+        assert (
+            hasattr(
+                self.menu_controller.parent_controller, "microscope_popup_controller"
+            )
+            is False
+        )
+
+    def test_toggle_save(self):
+        class MockWidget:
+            def __int__(self):
+                self.value = False
+
+            def set(self, value):
+                self.value = value
+
+            def get(self):
+                return self.value
+
+        channel_tab_controller = MagicMock()
+        self.menu_controller.parent_controller.channels_tab_controller = (
+            channel_tab_controller
+        )
+        channel_tab_controller.timepoint_vals = {"is_save": MockWidget()}
+        temp = self.menu_controller.view.settings.channels_tab.stack_timepoint_frame
+        temp.save_data.get = MagicMock(return_value=False)
+        self.menu_controller.toggle_save()
+        assert channel_tab_controller.timepoint_vals["is_save"].get() is True
+
+        temp = self.menu_controller.view.settings.channels_tab.stack_timepoint_frame
+        temp.save_data.get = MagicMock(return_value=True)
+        self.menu_controller.toggle_save()
+        assert channel_tab_controller.timepoint_vals["is_save"].get() is False
+
+    def test_stage_movement(self):
+        # TODO: DummyController does not have a stage controller.
+        pass
+
+    def test_switch_tabs(self):
+        for i in range(1, 4):
+            self.menu_controller.switch_tabs(window="left", tab=i)
+            assert (
+                self.menu_controller.parent_controller.view.settings.index("current")
+                == i - 1
+            )
+
+    @patch("src.navigate.controller.sub_controllers.menus.platform.system")
+    @patch("src.navigate.controller.sub_controllers.menus.subprocess.check_call")
+    def test_open_folder(self, mock_check_call, mock_system):
+        mock_system.return_value = "Darwin"
+        self.menu_controller.open_folder("test_path")
+        mock_check_call.assert_called_once_with(["open", "--", "test_path"])
+
+        mock_check_call.reset_mock()
+        mock_system.return_value = "Windows"
+        self.menu_controller.open_folder("test_path")
+        mock_check_call.assert_called_once_with(["explorer", "test_path"])
+
+        mock_check_call.reset_mock()
+        mock_system.return_value = "Linux"
+        self.menu_controller.open_folder("test_path")
+        self.assertEqual(mock_check_call.call_count, 0)
+
+    @patch("src.navigate.controller.sub_controllers.menus.os.path.join")
+    def test_open_log_files(self, mock_join):
+        with patch.object(self.menu_controller, "open_folder") as mock_open_folder:
+            mock_join.return_value = "joined_path"
+            self.menu_controller.open_log_files()
+            mock_open_folder.assert_called_once_with("joined_path")
+
+    @patch("src.navigate.controller.sub_controllers.menus.os.path.join")
+    def test_open_configuration_files(self, mock_join):
+        with patch.object(self.menu_controller, "open_folder") as mock_open_folder:
+            mock_join.return_value = "joined_path"
+            self.menu_controller.open_configuration_files()
+            mock_open_folder.assert_called_once_with("joined_path")

test/controller/sub_controllers/test_multiposition.py

@@ -0,0 +1,171 @@
+# Copyright (c) 2021-2025  The University of Texas Southwestern Medical Center.
+# All rights reserved.
+
+# Redistribution and use in source and binary forms, with or without
+# modification, are permitted for academic and research use only
+# (subject to the limitations in the disclaimer below)
+# provided that the following conditions are met:
+
+#      * Redistributions of source code must retain the above copyright notice,
+#      this list of conditions and the following disclaimer.
+
+#      * Redistributions in binary form must reproduce the above copyright
+#      notice, this list of conditions and the following disclaimer in the
+#      documentation and/or other materials provided with the distribution.
+
+#      * Neither the name of the copyright holders nor the names of its
+#      contributors may be used to endorse or promote products derived from this
+#      software without specific prior written permission.
+
+# NO EXPRESS OR IMPLIED LICENSES TO ANY PARTY'S PATENT RIGHTS ARE GRANTED BY
+# THIS LICENSE. THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND
+# CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT
+# LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
+# PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT HOLDER OR
+# CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL,
+# EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO,
+# PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR
+# BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER
+# IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE)
+# ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE
+# POSSIBILITY OF SUCH DAMAGE.
+#
+
+# Standard library imports
+
+# Third party imports
+import pytest
+import unittest
+from unittest.mock import MagicMock, patch
+import pandas as pd
+
+# Local application imports
+from navigate.controller.sub_controllers.multiposition import MultiPositionController
+
+
+@pytest.fixture
+def multiposition_controller(dummy_controller):
+    # Create a copy/clone of the dummy_controller to avoid side effects
+    isolated_controller = MagicMock()
+    isolated_controller.configuration = dummy_controller.configuration
+
+    # Create a mock pt attribute for the multiposition_tab
+    isolated_controller.view.settings.multiposition_tab.pt = MagicMock()
+    isolated_controller.view.settings.multiposition_tab.pt.model = MagicMock()
+    isolated_controller.view.settings.multiposition_tab.pt.model.df = pd.DataFrame()
+
+    # Add other required mock attributes and methods
+    isolated_controller.view.settings.multiposition_tab.pt.redraw = MagicMock()
+    isolated_controller.view.settings.multiposition_tab.pt.tableChanged = MagicMock()
+    isolated_controller.view.settings.multiposition_tab.pt.resetColors = MagicMock()
+    isolated_controller.view.settings.multiposition_tab.pt.update_rowcolors = (
+        MagicMock()
+    )
+
+    # Mock the master and tiling buttons
+    isolated_controller.view.settings.multiposition_tab.master = MagicMock()
+    isolated_controller.view.settings.multiposition_tab.master.tiling_buttons = (
+        MagicMock()
+    )
+    isolated_controller.view.settings.multiposition_tab.master.tiling_buttons.buttons = {
+        "tiling": MagicMock(),
+        "save_data": MagicMock(),
+        "load_data": MagicMock(),
+        "eliminate_tiles": MagicMock(),
+    }
+
+    # This is the important part - configure the stage axes
+    isolated_controller.configuration_controller = MagicMock()
+    isolated_controller.configuration_controller.stage_axes = [
+        "x",
+        "y",
+        "z",
+        "theta",
+        "f",
+    ]
+
+    return MultiPositionController(
+        isolated_controller.view.settings.multiposition_tab, isolated_controller
+    )
+
+
+@patch("navigate.controller.sub_controllers.multiposition.filedialog.askopenfilenames")
+@patch("navigate.controller.sub_controllers.multiposition.yaml.safe_load")
+@patch("builtins.open", new_callable=unittest.mock.mock_open, read_data="dummy content")
+def test_load_positions_yaml(
+    mock_file, mock_safe_load, mock_askopen, multiposition_controller
+):
+    """Test loading positions from YAML"""
+    controller = multiposition_controller
+    table = controller.table
+
+    mock_askopen.return_value = ("dummy_file.yml",)
+    mock_safe_load.return_value = [
+        ["X", "Y", "Z", "THETA", "F"],
+        [0, 0, 0, 0, 0],
+        [100, 200, 300, 400, 500],
+    ]
+
+    controller.load_positions()
+
+    mock_file.assert_called_once_with("dummy_file.yml", "r")
+
+    expected = pd.DataFrame(
+        [[0, 0, 0, 0, 0], [100, 200, 300, 400, 500]],
+        columns=["X", "Y", "Z", "THETA", "F"],
+    )
+    pd.testing.assert_frame_equal(table.model.df, expected)
+
+
+@patch("navigate.controller.sub_controllers.multiposition.filedialog.askopenfilenames")
+@patch("navigate.controller.sub_controllers.multiposition.pd.read_csv")
+def test_load_positions_csv(mock_read_csv, mock_askopen, multiposition_controller):
+    """Test loading positions from CSV"""
+    controller = multiposition_controller
+    table = controller.table
+
+    mock_askopen.return_value = ("dummy_file.csv",)
+    mock_read_csv.return_value = pd.DataFrame(
+        {"X": [1, 2], "Y": [3, 4], "Z": [5, 6], "THETA": [0, 0], "F": [0, 0]}
+    )
+
+    controller.load_positions()
+
+    expected = pd.DataFrame(
+        {"X": [1, 2], "Y": [3, 4], "Z": [5, 6], "THETA": [0, 0], "F": [0, 0]}
+    )
+    pd.testing.assert_frame_equal(table.model.df, expected)
+
+
+@patch("navigate.controller.sub_controllers.multiposition.filedialog.asksaveasfilename")
+@patch("navigate.controller.sub_controllers.multiposition.save_yaml_file")
+def test_export_positions_yaml(mock_save_yaml, mock_asksave, multiposition_controller):
+    """Test exporting positions to YAML"""
+    controller = multiposition_controller
+    table = controller.table
+
+    table.model.df = pd.DataFrame(
+        {"X": [1, 2], "Y": [3, 4], "Z": [5, 6], "THETA": [0, 0], "F": [0, 0]}
+    )
+    mock_asksave.return_value = "/tmp/output.yml"
+
+    controller.export_positions()
+    mock_save_yaml.assert_called_once()
+
+
+@patch("navigate.controller.sub_controllers.multiposition.filedialog.asksaveasfilename")
+def test_export_positions_csv(mock_asksave, multiposition_controller):
+    """Test exporting positions to CSV"""
+    controller = multiposition_controller
+    table = controller.table
+
+    df = pd.DataFrame(
+        {"X": [1, 2], "Y": [3, 4], "Z": [5, 6], "THETA": [0, 0], "F": [0, 0]}
+    )
+    table.model.df = df
+    table.model.df.to_csv = MagicMock()
+
+    mock_asksave.return_value = "/tmp/output.csv"
+
+    controller.export_positions()
+    table.model.df.to_csv.assert_called_once_with("/tmp/output.csv", index=False)

test/controller/sub_controllers/test_stages.py

@@ -0,0 +1,376 @@
+# Copyright (c) 2021-2025  The University of Texas Southwestern Medical Center.
+# All rights reserved.
+
+# Redistribution and use in source and binary forms, with or without
+# modification, are permitted for academic and research use only (subject to the
+# limitations in the disclaimer below) provided that the following conditions are met:
+
+#      * Redistributions of source code must retain the above copyright notice,
+#      this list of conditions and the following disclaimer.
+
+#      * Redistributions in binary form must reproduce the above copyright
+#      notice, this list of conditions and the following disclaimer in the
+#      documentation and/or other materials provided with the distribution.
+
+#      * Neither the name of the copyright holders nor the names of its
+#      contributors may be used to endorse or promote products derived from this
+#      software without specific prior written permission.
+
+# NO EXPRESS OR IMPLIED LICENSES TO ANY PARTY'S PATENT RIGHTS ARE GRANTED BY
+# THIS LICENSE. THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND
+# CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT
+# LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
+# PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT HOLDER OR
+# CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL,
+# EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO,
+# PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR
+# BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER
+# IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE)
+# ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE
+# POSSIBILITY OF SUCH DAMAGE.
+#
+
+import pytest
+from unittest.mock import MagicMock, call
+import numpy as np
+
+AXES = ["x", "y", "z", "theta", "f"]
+CAXES = ["xy", "z", "theta", "f"]
+
+
+def pos_dict(v, axes=AXES):
+    return {k: v for k in axes}
+
+
+@pytest.fixture
+def stage_controller(dummy_controller):
+    from navigate.controller.sub_controllers.stages import StageController
+
+    dummy_controller.camera_view_controller = MagicMock()
+
+    stage_controller = StageController(
+        dummy_controller.view.settings.stage_control_tab,
+        dummy_controller,
+    )
+
+    dummy_controller.view.settings.stage_control_tab.focus_get = MagicMock(
+        return_value=True
+    )
+
+    return stage_controller
+
+
+# test before set position variables to MagicMock()
+def test_set_position(stage_controller):
+
+    widgets = stage_controller.view.get_widgets()
+    vals = {}
+    for axis in AXES:
+        widgets[axis].widget.trigger_focusout_validation = MagicMock()
+        vals[axis] = np.random.randint(0, 9)
+
+    stage_controller.view.get_widgets = MagicMock(return_value=widgets)
+    stage_controller.show_verbose_info = MagicMock()
+    position = {
+        "x": np.random.random(),
+        "y": np.random.random(),
+        "z": np.random.random(),
+    }
+    stage_controller.set_position(position)
+    for axis in position.keys():
+        assert float(stage_controller.widget_vals[axis].get()) == position[axis]
+        assert widgets[axis].widget.trigger_focusout_validation.called
+        assert stage_controller.stage_setting_dict[axis] == position.get(axis, 0)
+    stage_controller.show_verbose_info.assert_has_calls(
+        [call("Stage position changed"), call("Set stage position")]
+    )
+
+
+def test_set_position_silent(stage_controller):
+
+    widgets = stage_controller.view.get_widgets()
+    vals = {}
+    for axis in AXES:
+        widgets[axis].widget.trigger_focusout_validation = MagicMock()
+        vals[axis] = np.random.randint(0, 9)
+
+    stage_controller.view.get_widgets = MagicMock(return_value=widgets)
+    stage_controller.show_verbose_info = MagicMock()
+    position = {
+        "x": np.random.random(),
+        "y": np.random.random(),
+        "z": np.random.random(),
+    }
+    stage_controller.set_position_silent(position)
+    for axis in position.keys():
+        assert float(stage_controller.widget_vals[axis].get()) == position[axis]
+        widgets[axis].widget.trigger_focusout_validation.assert_called_once()
+        assert stage_controller.stage_setting_dict[axis] == position.get(axis, 0)
+    stage_controller.show_verbose_info.assert_has_calls([call("Set stage position")])
+    assert (
+        call("Stage position changed")
+        not in stage_controller.show_verbose_info.mock_calls
+    )
+
+
+@pytest.mark.parametrize(
+    "flip_x, flip_y",
+    [(False, False), (True, False), (True, True), (False, True), (True, True)],
+)
+def test_stage_key_press(stage_controller, flip_x, flip_y):
+    microscope_name = (
+        stage_controller.parent_controller.configuration_controller.microscope_name
+    )
+    stage_config = stage_controller.parent_controller.configuration["configuration"][
+        "microscopes"
+    ][microscope_name]["stage"]
+    stage_config["flip_x"] = flip_x
+    stage_config["flip_y"] = flip_y
+    stage_controller.initialize()
+    x = round(np.random.random(), 1)
+    y = round(np.random.random(), 1)
+    increment = round(np.random.random() + 1, 1)
+    stage_controller.widget_vals["xy_step"].get = MagicMock(return_value=increment)
+    stage_controller.widget_vals["x"].get = MagicMock(return_value=x)
+    stage_controller.widget_vals["x"].set = MagicMock()
+    stage_controller.widget_vals["y"].get = MagicMock(return_value=y)
+    stage_controller.widget_vals["y"].set = MagicMock()
+    event = MagicMock()
+
+    axes_map = {"w": "y", "a": "x", "s": "y", "d": "x"}
+
+    for char, xs, ys in zip(
+        ["w", "a", "s", "d"],
+        [0, -increment, 0, increment],
+        [increment, 0, -increment, 0],
+    ):
+        event.char = char
+        # <a> instead of <Control+a>
+        event.state = 0
+        axis = axes_map[char]
+        if axis == "x":
+            temp = x + xs * (-1 if flip_x else 1)
+        else:
+            temp = y + ys * (-1 if flip_y else 1)
+        stage_controller.stage_key_press(event)
+        stage_controller.widget_vals[axis].set.assert_called_once_with(temp)
+        stage_controller.widget_vals[axis].set.reset_mock()
+        stage_controller.widget_vals[axis].get.reset_mock()
+        stage_controller.widget_vals["xy_step"].get.reset_mock()
+
+    stage_config["flip_x"] = False
+    stage_config["flip_y"] = False
+
+
+def test_get_position(stage_controller):
+    import tkinter as tk
+
+    vals = {}
+    for axis in AXES:
+        vals[axis] = np.random.randint(0, 9)
+        stage_controller.widget_vals[axis].get = MagicMock(return_value=vals[axis])
+
+    step_vals = {}
+    for axis in CAXES:
+        step_vals[axis] = np.random.randint(1, 9)
+        stage_controller.widget_vals[axis + "_step"].get = MagicMock(
+            return_value=step_vals[axis]
+        )
+
+    stage_controller.position_min = pos_dict(0)
+    stage_controller.position_max = pos_dict(10)
+    position = stage_controller.get_position()
+    assert position == {k: vals[k] for k in AXES}
+
+    stage_controller.position_min = pos_dict(2)
+
+    vals = {}
+    for axis in AXES:
+        vals[axis] = np.random.choice(
+            np.concatenate((np.arange(-9, 0), np.arange(10, 20)))
+        )
+        stage_controller.widget_vals[axis].get = MagicMock(return_value=vals[axis])
+
+    position = stage_controller.get_position()
+    assert position is None
+
+    stage_controller.widget_vals["x"].get.side_effect = tk.TclError
+    position = stage_controller.get_position()
+    assert position is None
+
+
+@pytest.mark.parametrize(
+    "flip_x, flip_y, flip_z",
+    [
+        (False, False, False),
+        (True, False, False),
+        (True, True, False),
+        (False, True, True),
+        (True, True, True),
+    ],
+)
+def test_up_btn_handler(stage_controller, flip_x, flip_y, flip_z):
+    microscope_name = (
+        stage_controller.parent_controller.configuration_controller.microscope_name
+    )
+    stage_config = stage_controller.parent_controller.configuration["configuration"][
+        "microscopes"
+    ][microscope_name]["stage"]
+    stage_config["flip_x"] = flip_x
+    stage_config["flip_y"] = flip_y
+    stage_config["flip_z"] = flip_z
+    stage_controller.initialize()
+    flip_flags = (
+        stage_controller.parent_controller.configuration_controller.stage_flip_flags
+    )
+
+    vals = {}
+    for axis in AXES:
+        vals[axis] = np.random.randint(1, 9)
+        stage_controller.widget_vals[axis].get = MagicMock(return_value=vals[axis])
+        stage_controller.widget_vals[axis].set = MagicMock()
+
+    step_vals = {}
+    for axis in CAXES:
+        step_vals[axis] = np.random.randint(1, 9)
+        stage_controller.widget_vals[axis + "_step"].get = MagicMock(
+            return_value=step_vals[axis]
+        )
+
+    stage_controller.position_max = pos_dict(10)
+
+    # Test for each axis
+    for axis in AXES:
+        pos = stage_controller.widget_vals[axis].get()
+        if axis == "x" or axis == "y":
+            step = stage_controller.widget_vals["xy_step"].get()
+        else:
+            step = stage_controller.widget_vals[axis + "_step"].get()
+        temp = pos + step * (-1 if flip_flags[axis] else 1)
+        if temp > stage_controller.position_max[axis]:
+            temp = stage_controller.position_max[axis]
+        stage_controller.up_btn_handler(axis)()
+        stage_controller.widget_vals[axis].set.assert_called_once_with(temp)
+
+    # Test for out of limit condition
+    for axis in AXES:
+        stage_controller.widget_vals[axis].set.reset_mock()
+        stage_controller.widget_vals[axis].get.return_value = 10
+        stage_controller.up_btn_handler(axis)()
+        if flip_flags[axis] is False:
+            stage_controller.widget_vals[axis].set.assert_not_called()
+
+    stage_config["flip_x"] = False
+    stage_config["flip_y"] = False
+    stage_config["flip_z"] = False
+
+
+@pytest.mark.parametrize(
+    "flip_x, flip_y, flip_z",
+    [
+        (False, False, False),
+        (True, False, False),
+        (True, True, False),
+        (False, True, True),
+        (True, True, True),
+    ],
+)
+def test_down_btn_handler(stage_controller, flip_x, flip_y, flip_z):
+    microscope_name = (
+        stage_controller.parent_controller.configuration_controller.microscope_name
+    )
+    stage_config = stage_controller.parent_controller.configuration["configuration"][
+        "microscopes"
+    ][microscope_name]["stage"]
+    stage_config["flip_x"] = flip_x
+    stage_config["flip_y"] = flip_y
+    stage_config["flip_z"] = flip_z
+    stage_controller.initialize()
+    flip_flags = (
+        stage_controller.parent_controller.configuration_controller.stage_flip_flags
+    )
+    vals = {}
+    for axis in AXES:
+        vals[axis] = np.random.randint(1, 9)
+        stage_controller.widget_vals[axis].get = MagicMock(return_value=vals[axis])
+        stage_controller.widget_vals[axis].set = MagicMock()
+
+    step_vals = {}
+    for axis in CAXES:
+        step_vals[axis] = np.random.randint(1, 9)
+        stage_controller.widget_vals[axis + "_step"].get = MagicMock(
+            return_value=step_vals[axis]
+        )
+
+    stage_controller.position_min = pos_dict(0)
+
+    # Test for each axis
+    for axis in AXES:
+        pos = stage_controller.widget_vals[axis].get()
+        if axis == "x" or axis == "y":
+            step = stage_controller.widget_vals["xy_step"].get()
+        else:
+            step = stage_controller.widget_vals[axis + "_step"].get()
+        temp = pos - step * (-1 if flip_flags[axis] else 1)
+        if temp < stage_controller.position_min[axis]:
+            temp = stage_controller.position_min[axis]
+        stage_controller.down_btn_handler(axis)()
+        stage_controller.widget_vals[axis].set.assert_called_once_with(temp)
+
+    # Test for out of limit condition
+    for axis in ["x", "y", "z", "theta", "f"]:
+        stage_controller.widget_vals[axis].set.reset_mock()
+        stage_controller.widget_vals[axis].get.return_value = 0
+        stage_controller.down_btn_handler(axis)()
+        if flip_flags[axis] is False:
+            stage_controller.widget_vals[axis].set.assert_not_called()
+
+    stage_config["flip_x"] = False
+    stage_config["flip_y"] = False
+    stage_config["flip_z"] = False
+
+
+def test_stop_button_handler(stage_controller):
+
+    stage_controller.view.after = MagicMock()
+
+    stage_controller.stop_button_handler()
+
+    stage_controller.view.after.assert_called_once()
+
+
+def test_position_callback(stage_controller):
+
+    stage_controller.show_verbose_info = MagicMock()
+
+    stage_controller.view.after = MagicMock()
+
+    vals = {}
+    widgets = stage_controller.view.get_widgets()
+    for axis in AXES:
+        vals[axis] = np.random.randint(1, 9)
+        stage_controller.widget_vals[axis].get = MagicMock(return_value=vals[axis])
+        stage_controller.widget_vals[axis].set = MagicMock()
+        widgets[axis].widget.set(vals[axis])
+        widgets[axis].widget.trigger_focusout_validation = MagicMock()
+
+    stage_controller.position_min = pos_dict(0)
+    stage_controller.position_max = pos_dict(10)
+    stage_controller.stage_setting_dict = {}
+
+    for axis in AXES:
+
+        callback = stage_controller.position_callback(axis)
+
+        # Test case 1: Position variable is within limits
+        widgets[axis].widget.get = MagicMock(return_value=vals[axis])
+        callback()
+        stage_controller.view.after.assert_called()
+        stage_controller.view.after.reset_mock()
+        assert stage_controller.stage_setting_dict[axis] == vals[axis]
+
+        # Test case 2: Position variable is outside limits
+        widgets[axis].widget.get = MagicMock(return_value=11)
+        callback()
+        stage_controller.view.after.assert_called_once()
+        stage_controller.view.after.reset_mock()

test/controller/sub_controllers/test_tiling.py

@@ -0,0 +1,215 @@
+import random
+import pytest
+
+
+@pytest.fixture(scope="module")
+def tiling_wizard_controller(dummy_view, dummy_controller):
+    from navigate.view.popups.tiling_wizard_popup2 import TilingWizardPopup
+    from navigate.controller.sub_controllers.tiling import (
+        TilingWizardController,
+    )
+
+    tiling_wizard = TilingWizardPopup(dummy_view)
+
+    class SubController:
+        def __init__(self):
+            self.parent_controller = dummy_controller
+
+    return TilingWizardController(tiling_wizard, SubController())
+
+
+def test_traces(tiling_wizard_controller):
+    """TODO: Find a way to access the actual lambda functions.
+
+    If we can, inspect.getsource(myfunc) should provide us the lambda definition.
+    """
+
+    def assert_one_trace(var):
+        tinfo = var.trace_info()
+        assert len(tinfo) >= 1
+        assert tinfo[0][0][0] == "write"
+        assert "lambda" in tinfo[0][1]
+
+    for ax in ["x", "y", "z", "f"]:
+        # self.variables["x_start"], etc. should all be bound to two lambda functions
+        # calling calculate_distance() and update_fov()
+        for bound in ["start", "end"]:
+            tinfo = tiling_wizard_controller.variables[f"{ax}_{bound}"].trace_info()
+            assert len(tinfo) >= 1
+            for ti in tinfo:
+                assert ti[0][0] == "write"
+                assert "lambda" in ti[1]
+
+        # fov should be bound to one lambda, calling calculate_tiles()
+        assert_one_trace(tiling_wizard_controller.variables[f"{ax}_fov"])
+
+        # dist should be bound to one lambda, calling calculate_tiles()
+        assert_one_trace(tiling_wizard_controller.variables[f"{ax}_dist"])
+
+    # Special cases
+    assert_one_trace(tiling_wizard_controller.variables["percent_overlap"])
+    assert_one_trace(
+        tiling_wizard_controller.cam_settings_widgets["FOV_X"].get_variable()
+    )
+    assert_one_trace(
+        tiling_wizard_controller.cam_settings_widgets["FOV_Y"].get_variable()
+    )
+    assert_one_trace(
+        tiling_wizard_controller.stack_acq_widgets["start_position"].get_variable()
+    )
+    assert_one_trace(
+        tiling_wizard_controller.stack_acq_widgets["end_position"].get_variable()
+    )
+    # Channels tab controller binds these a bunch
+    # assert_one_trace(
+    #     tiling_wizard_controller.stack_acq_widgets["start_focus"].get_variable()
+    # )
+    # assert_one_trace(
+    #     tiling_wizard_controller.stack_acq_widgets["end_focus"].get_variable()
+    # )
+
+
+def test_update_total_tiles(tiling_wizard_controller):
+    tiling_wizard_controller.update_total_tiles()
+
+    assert True
+
+
+@pytest.mark.parametrize("axis", ["x", "y", "z", "f"])
+def test_calculate_tiles(tiling_wizard_controller, axis):
+    from navigate.tools.multipos_table_tools import calc_num_tiles
+
+    ov, dist, fov = random.random(), random.random() * 100, random.random() * 10
+    tiling_wizard_controller._percent_overlap = ov * 100
+    tiling_wizard_controller.variables[f"{axis}_dist"].set(dist)
+    tiling_wizard_controller.variables[f"{axis}_fov"].set(fov)
+    tiling_wizard_controller.calculate_tiles(axis)
+
+    if axis == "x" or axis == "y":
+        dist += fov
+
+    assert int(
+        tiling_wizard_controller.variables[f"{axis}_tiles"].get()
+    ) == calc_num_tiles(dist, ov, fov)
+
+
+@pytest.mark.parametrize("axis", ["x", "y", "z", "f"])
+def test_calculate_distance(tiling_wizard_controller, axis):
+    start, end = random.random() * 10, random.random() * 100
+    tiling_wizard_controller.variables[axis + "_start"].set(start)
+    tiling_wizard_controller.variables[axis + "_end"].set(end)
+    tiling_wizard_controller.calculate_distance(axis)
+    assert float(tiling_wizard_controller.variables[axis + "_dist"].get()) == abs(
+        start - end
+    )
+
+
+def test_update_overlap(tiling_wizard_controller):
+    tiling_wizard_controller.variables["percent_overlap"].set("")
+    tiling_wizard_controller.update_overlap()
+    tiling_wizard_controller.variables["percent_overlap"].set("10")
+    tiling_wizard_controller.update_overlap()
+
+    assert True
+
+
+@pytest.mark.parametrize("axis", ["x", "y", "z", "f"])
+def test_update_fov(tiling_wizard_controller, axis):
+    import random
+    from navigate.tools.multipos_table_tools import sign
+
+    if axis == "y":
+        tiling_wizard_controller.cam_settings_widgets["FOV_X"].set(
+            int(random.random() * 1000)
+        )
+        tiling_wizard_controller.variables["x_start"].set(random.random() * 10)
+        tiling_wizard_controller.variables["x_end"].set(random.random() * 1000)
+        var = float(
+            tiling_wizard_controller.cam_settings_widgets["FOV_X"].get()
+        ) * sign(
+            float(tiling_wizard_controller.variables["x_end"].get())
+            - float(tiling_wizard_controller.variables["x_start"].get())
+        )
+    elif axis == "x":
+        tiling_wizard_controller.cam_settings_widgets["FOV_Y"].set(
+            int(random.random() * 1000)
+        )
+        tiling_wizard_controller.variables["y_start"].set(random.random() * 10)
+        tiling_wizard_controller.variables["y_end"].set(random.random() * 1000)
+        var = float(
+            tiling_wizard_controller.cam_settings_widgets["FOV_Y"].get()
+        ) * sign(
+            float(tiling_wizard_controller.variables["y_end"].get())
+            - float(tiling_wizard_controller.variables["y_start"].get())
+        )
+    elif axis == "z":
+        tiling_wizard_controller.stack_acq_widgets["start_position"].set(
+            random.random() * 10
+        )
+        tiling_wizard_controller.stack_acq_widgets["end_position"].set(
+            random.random() * 1000
+        )
+        var = float(
+            tiling_wizard_controller.stack_acq_widgets["end_position"].get()
+        ) - float(tiling_wizard_controller.stack_acq_widgets["start_position"].get())
+    elif axis == "f":
+        tiling_wizard_controller.stack_acq_widgets["start_focus"].set(
+            random.random() * 10
+        )
+        tiling_wizard_controller.stack_acq_widgets["end_focus"].set(
+            random.random() * 1000
+        )
+        var = float(
+            tiling_wizard_controller.stack_acq_widgets["end_focus"].get()
+        ) - float(tiling_wizard_controller.stack_acq_widgets["start_focus"].get())
+    tiling_wizard_controller.update_fov(axis)
+
+    assert float(tiling_wizard_controller.variables[f"{axis}_fov"].get()) == abs(var)
+
+
+def test_set_table(tiling_wizard_controller):
+    # from navigate.tools.multipos_table_tools import compute_tiles_from_bounding_box
+    tiling_wizard_controller.set_table()
+
+    x_start = float(tiling_wizard_controller.variables["x_start"].get())
+    x_stop = float(tiling_wizard_controller.variables["x_end"].get())
+
+    y_start = float(tiling_wizard_controller.variables["y_start"].get())
+    y_stop = float(tiling_wizard_controller.variables["y_end"].get())
+
+    # shift z by coordinate origin of local z-stack
+    z_start = float(tiling_wizard_controller.variables["z_start"].get()) - float(
+        tiling_wizard_controller.stack_acq_widgets["start_position"].get()
+    )
+    z_stop = float(tiling_wizard_controller.variables["z_end"].get()) - float(
+        tiling_wizard_controller.stack_acq_widgets["end_position"].get()
+    )
+
+    # Default to fixed theta
+    r_start = tiling_wizard_controller.stage_position_vars["theta"].get()
+    r_stop = tiling_wizard_controller.stage_position_vars["theta"].get()
+
+    f_start = float(tiling_wizard_controller.variables["f_start"].get()) - float(
+        tiling_wizard_controller.stack_acq_widgets["start_focus"].get()
+    )
+    f_stop = float(tiling_wizard_controller.variables["f_end"].get()) - float(
+        tiling_wizard_controller.stack_acq_widgets["end_focus"].get()
+    )
+
+    # for consistency, always go from low to high
+    def sort_vars(a, b):
+        if a > b:
+            return b, a
+        return a, b
+
+    x_start, x_stop = sort_vars(x_start, x_stop)
+    y_start, y_stop = sort_vars(y_start, y_stop)
+    z_start, z_stop = sort_vars(z_start, z_stop)
+    r_start, r_stop = sort_vars(r_start, r_stop)
+    f_start, f_stop = sort_vars(f_start, f_stop)
+
+    assert tiling_wizard_controller.multipoint_table.model.df["X"].min() == x_start
+    assert tiling_wizard_controller.multipoint_table.model.df["Y"].min() == y_start
+    assert tiling_wizard_controller.multipoint_table.model.df["Z"].min() == z_start
+    assert tiling_wizard_controller.multipoint_table.model.df["THETA"].min() == r_start
+    assert tiling_wizard_controller.multipoint_table.model.df["F"].min() == f_start

test/controller/sub_controllers/test_waveform_popup.py

@@ -0,0 +1,178 @@
+import pytest
+import random
+from unittest.mock import MagicMock
+
+
+@pytest.fixture(scope="module")
+def waveform_popup_controller(dummy_view, dummy_controller):
+    from navigate.controller.sub_controllers.waveform_popup import (
+        WaveformPopupController,
+    )
+    from navigate.view.popups.waveform_parameter_popup_window import (
+        WaveformParameterPopupWindow,
+    )
+
+    waveform_constants_popup = WaveformParameterPopupWindow(
+        dummy_view, dummy_controller.configuration_controller
+    )
+
+    return WaveformPopupController(
+        waveform_constants_popup,
+        dummy_controller,
+        dummy_controller.waveform_constants_path,
+    )
+
+
+def test_populate_experiment_values(waveform_popup_controller):
+    exp_dict = waveform_popup_controller.parent_controller.configuration["experiment"][
+        "MicroscopeState"
+    ]
+    resolution = exp_dict["microscope_name"]
+    zoom = exp_dict["zoom"]
+    waveform_constants = waveform_popup_controller.parent_controller.configuration[
+        "waveform_constants"
+    ]
+    widgets = waveform_popup_controller.view.get_widgets()
+
+    def assert_widget_values():
+        resolution = exp_dict["microscope_name"]
+        zoom = exp_dict["zoom"]
+        assert widgets["Mode"].get() == resolution
+        assert widgets["Mag"].get() == zoom
+
+        # remote focus
+        remote_focus_dict = waveform_constants["remote_focus_constants"][resolution][
+            zoom
+        ]
+        for k in remote_focus_dict.keys():
+            assert widgets[k + " Amp"].get() == remote_focus_dict[k]["amplitude"]
+            assert widgets[k + " Off"].get() == remote_focus_dict[k]["offset"]
+
+        # galvo
+        galvo_dict = waveform_constants["galvo_constants"]
+        for g in galvo_dict.keys():
+            if resolution in [galvo_dict[g].keys()]:
+                galvo_info = galvo_dict[g][resolution][zoom]
+                assert widgets[g + " Amp"].get() == galvo_info["amplitude"]
+                assert widgets[g + " Off"].get() == galvo_info["offset"]
+
+        # delay, fly back time, settle duraation, smoothing
+        assert widgets["Delay"].get() == str(
+            waveform_constants["other_constants"]["remote_focus_delay"]
+        )
+        assert widgets["Ramp_falling"].get() == str(
+            waveform_constants["other_constants"]["remote_focus_ramp_falling"]
+        )
+        assert widgets["Duty"].get() == str(
+            waveform_constants["other_constants"]["remote_focus_settle_duration"]
+        )
+        assert widgets["Smoothing"].get() == str(
+            waveform_constants["other_constants"]["percent_smoothing"]
+        )
+
+    # default values
+    waveform_popup_controller.populate_experiment_values()
+    assert_widget_values()
+
+    # change resolution and/or zoom
+    for microscope_name in waveform_constants["remote_focus_constants"].keys():
+        for z in waveform_constants["remote_focus_constants"][microscope_name].keys():
+            exp_dict["microscope_name"] = microscope_name
+            exp_dict["zoom"] = z
+            waveform_popup_controller.populate_experiment_values()
+            assert_widget_values()
+
+    exp_dict["microscope_name"] = resolution
+    exp_dict["zoom"] = zoom
+    waveform_popup_controller.populate_experiment_values()
+    assert_widget_values()
+
+    # update waveform_constants
+    for k in waveform_constants["remote_focus_constants"][resolution][zoom].keys():
+        amplitude = round(random.random() * 5, 2)
+        offset = round(random.random() * 5, 2)
+        temp = waveform_constants["remote_focus_constants"][resolution][zoom][k]
+        temp["amplitude"] = amplitude
+        temp["offset"] = offset
+
+    # update galvo
+    for g in waveform_constants["galvo_constants"].keys():
+        amplitude = round(random.random() * 5, 2)
+        offset = round(random.random() * 5, 2)
+        temp = waveform_constants["galvo_constants"][g][resolution][zoom]
+        temp["amplitude"] = amplitude
+        temp["offset"] = offset
+
+    for k in [
+        "remote_focus_ramp_falling",
+        "remote_focus_settle_duration",
+        "percent_smoothing",
+        "remote_focus_delay",
+    ]:
+        waveform_constants["other_constants"][k] = round(random.random() * 100, 2)
+
+    waveform_popup_controller.populate_experiment_values(force_update=True)
+    assert_widget_values()
+
+
+def test_show_laser_info(waveform_popup_controller):
+    waveform_popup_controller.show_laser_info()
+    assert True
+
+
+def test_configure_widget_range(waveform_popup_controller):
+    waveform_popup_controller.configure_widget_range()
+    assert True
+
+
+def test_estimate_galvo_setting_empty_string(waveform_popup_controller):
+    """Test if the function returns without calling the camera setting controller."""
+    # Galvo name
+    galvo_name = "galvo_0"
+
+    # Mocked camera setting controller
+    waveform_popup_controller.parent_controller.camera_setting_controller = MagicMock()
+    waveform_popup_controller.parent_controller.camera_setting_controller.mode_widgets[
+        "Pixels"
+    ].get = MagicMock(return_value="")
+    waveform_popup_controller.parent_controller.camera_setting_controller.framerate_widgets[
+        "exposure_time"
+    ].get = MagicMock()
+
+    waveform_popup_controller.estimate_galvo_setting(galvo_name)
+    waveform_popup_controller.parent_controller.camera_setting_controller.framerate_widgets[
+        "exposure_time"
+    ].get.assert_not_called()
+
+
+def test_estimate_galvo_setting_with_string(waveform_popup_controller):
+    """Test if the function calls the camera setting controller."""
+    # Galvo name
+    galvo_name = "galvo_0"
+    number_of_pixels = 50
+
+    # Mocked camera setting controller
+    waveform_popup_controller.parent_controller.camera_setting_controller = MagicMock()
+    waveform_popup_controller.parent_controller.camera_setting_controller.mode_widgets[
+        "Pixels"
+    ].get = MagicMock(return_value=str(number_of_pixels))
+    waveform_popup_controller.parent_controller.camera_setting_controller.framerate_widgets[
+        "exposure_time"
+    ].get = MagicMock()
+
+    # Mocked model
+    waveform_popup_controller.parent_controller.model = MagicMock()
+    mock_model = waveform_popup_controller.parent_controller.model
+    mock_model.get_camera_line_interval_and_exposure_time = MagicMock(
+        return_value=(0.05, 50, 500)
+    )
+
+    # Mocked view
+    waveform_popup_controller.view = MagicMock()
+    waveform_popup_controller.view.inputs[galvo_name].widget.set = MagicMock()
+
+    # Call the function
+    waveform_popup_controller.estimate_galvo_setting(galvo_name)
+
+    # Check to see what the view was called with.
+    waveform_popup_controller.view.inputs[galvo_name].widget.set.assert_called_once()